AdipoGen Life Sciences

anti-Jagged-1 (human), mAb (J1G53-3) (FITC)

CHF 350.00

In stock

AG-20A-0049F-C05050 µgCHF 350.00

Figure 1: Flow cytometric analysis using anti-Jagged-1 (human), mAb (J1G53-3) (FITC) (Prod. No. AG-20A-0049F). A surface staining method was used to stain the normal human peripheral blood cells (CD4+ selections). An appropriate isotype control was used for FITC mouse IgG1.

Figure 2: FACS staining of human Lymphocyte(CD4+) using anti-Jagged-1 (human), mAb (J1G53-3) (FITC) (Prod. No. AG-20A-0049F).

Figure 3: Western blot analysis using anti-Jagged-1 (human), mAb (J1G53-3) (FITC) (Prod. No. AG-20A-0049F) at 1:1'000 dilution.
1: Human Jagged-1 Fc fusion protein.
2. Transfected human Jagged-1 cell lysate (HEK 293).
3. Mock transfected HEK 293 cell lysate.

Figure 4: SPR analysis of the interaction between anti-Jagged-1 (human), mAb (J1G53-3) derivative hJagged1-Fc fusion protein. SPR response was taken when 13, 6.5, 3.25, 1.625, and 0.8125nM of J1G53-3 were injected onto the hJagged1-Fc fusion protein. J1G53-3 exhibited the highest binding affinity, and its property and binding ability was studied in detail by spectroscopy and surface Plasmon resonance (SPR). The result acquired through SPR was as follows: Kd value was1.08nM and value of chi-square was 0.0237 through SPR analysis.

Specifications / Handling

More Information
Product Details
Synonyms	Protein Jagged-1; CD339; JAG1
Product Type	Monoclonal Antibody
Properties
Clone	J1G53-3
Isotype	Mouse IgG1κ
Immunogen/Antigen	Recombinant human Jagged-1.
Label/Conjugates	FITC
Application	Flow Cytometry: (1:20-1:100) Western blot: (1:500-1:5'000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature). Optimal conditions should be determined individually for each application.
Crossreactivity	Human
Specificity	Recognizes human Jagged-1. Detects a band of ~180kDa by Western blot.
Purity Detail	Protein G-affinity purified.
Concentration	Lot dependent.
Formulation	Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
Isotype Negative Control	Mouse IgG1 Isotype Control (FITC)
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Use/Stability	Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

Jagged-1 is a ligand for multiple Notch receptors and involved in the mediation of Notch signaling. It is involved in cell-fate decisions during hematopoiesis. Is involved in early and late stages of mammalian cardiovascular development. Inhibits myoblast differentiation. Enhances fibroblast growth factor-induced angiogenesis (in vitro). Defects in Jagged-1 are the cause of Alagille syndrome type 1 (ALGS1) and tetralogy of Fallot (TOF).

Product References

Preclinical activity of the repurposed drug auranofin in classical Hodgkin lymphoma: M. Celegato, et al., Blood 126, 1394 (2015)

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