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AdipoGen Life Sciences
anti-DLK1 (mouse), mAb (PF105B)
270
CHF
CHF 270.00
In stock
AG-20A-0057-C05050 µgCHF 270.00
AG-20A-0057-C100100 µgCHF 360.00
Figure 1: Western blot analysis using anti-DLK1 (mouse), mAb (PF105B) (Prod. No. AG-20A-0057) at 1:2'000 dilution.
A)
1: Mouse DLK1 Fc-protein.
2: Human DLK1 Fc-protein.
3: Mouse DLL1 Fc-protein.
4: Mouse DLL4 Fc protein.
5: Mouse Jagged-1 Fc-protein.
6: Unrelated Fc-protein (negative control).
B)
1: Mouse DLK1 Fc-protein.
2: Mouse DLL1 Fc-protein.
3: Human DLK1 (ED) (FLAG®-tagged).
4: 3T3L1 cell lysate.
A)
1: Mouse DLK1 Fc-protein.
2: Human DLK1 Fc-protein.
3: Mouse DLL1 Fc-protein.
4: Mouse DLL4 Fc protein.
5: Mouse Jagged-1 Fc-protein.
6: Unrelated Fc-protein (negative control).
B)
1: Mouse DLK1 Fc-protein.
2: Mouse DLL1 Fc-protein.
3: Human DLK1 (ED) (FLAG®-tagged).
4: 3T3L1 cell lysate.
| Product Details | |
|---|---|
| Synonyms | Protein Delta Homolog 1; Fetal Antigen 1; Preadipocyte Factor 1; Pref-1; FA1 |
| Product Type | Monoclonal Antibody |
| Properties | |
| Clone | PF105B |
| Isotype | Rat IgG2aκ |
| Immunogen/Antigen | Recombinant mouse DLK1 (extracellular domain). |
| Application |
ELISA: (direct or indirect: 1:2'000-1:10'000) |
| Crossreactivity | Mouse |
| Specificity |
Recognizes mouse DLK1. Does not cross-react with human DLK1. |
| Purity Detail | Protein G-affinity purified. |
| Concentration | ~1mg/ml |
| Formulation | Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives. |
| Isotype Negative Control | |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
The transmembrane and secreted protein delta-like 1 homolog (DLK1; Protein delta homolog 1; Preadipocyte factor 1; Pref-1) belongs to the EGF-like family. It can be cleaved by a tumor necrosis factor-α converting enzyme (TACE) to release a soluble form. DLK1 plays important roles in regulating cell differentiation such as adipogenesis and osteogenesis. Membrane-bound DLK1 promotes hypertrophic myotube formation, whereas soluble DLK1 inhibits myotube formation. The soluble form of DLK1 inhibits preadipocyte differentiation, while membrane DLK1 represses preadipocyte proliferation. DLK1 is also associated with tumor invasion.
Product References
- Identification of a stroma-mediated Wnt/beta-catenin signal promoting self-renewal of hematopoietic stem cells in the stem cell niche: J.A. Kim, et al.; Stem Cells 27, 1318 (2009)
- Distinct hypoxic regulation of preadipocyte factor-1 (Pref-1) in preadipocytes and mature adipocytes: Y. Moon, et al.; BBA Mol. Cell Res. 1865, 334 (2018)
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