AdipoGen Life Sciences

anti-FTO, mAb (FT86-4)

CHF 270.00

In stock

AG-20A-0064-C05050 µgCHF 270.00

AG-20A-0064-C100100 µgCHF 350.00

Figure 1: Immunoprecipitation of recombinant human FTO using anti-FTO (human), mAb (FT86-4) (Prod. No. AG-20A-0064). Recombinant human FTO proteins at different concentrations were precipitated by FT86-4. The precipitated proteins were separated by SDS-PAGE, electroblotted, and visualized by western blot with rabbit anti-mouse FTO pAb (AG-25A-0089).

Figure 2: Western blot analysis using anti-FTO (human), mAb (FT86-4) (Prod. No. AG-20A-0064) at 1:2'000 dilution.
1: Human FTO (His-tagged) (100ng).
2: Mouse FTO (His-tagged) (100ng).
3: Rat FTO (His-tagged) (100ng).
4: HEK 293 cell lysate (100μg).
5: Human Calreticulin (His-tagged) (100ng) (negative control).

Specifications / Handling

More Information
Product Details
Synonyms	Fat Mass and Obesity-associated Protein; FATSO
Product Type	Monoclonal Antibody
Properties
Clone	FT86-4
Isotype	Mouse IgG1κ
Immunogen/Antigen	Recombinant human FTO.
Application	ELISA: (direct or indirect: 1:2'000-1:5'000) Immunoprecipitation: (1-5μl per sample in a final reaction volume of 500-1000μl) Western blot: (1:2'000-1:5'000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature). IHC: (paraffin sections 1:2'000) Optimal conditions should be determined individually for each application.
Crossreactivity	Human Mouse Rat
Specificity	Recognizes human, mouse and rat FTO.
Purity Detail	Protein G-affinity purified.
Concentration	1mg/ml
Formulation	Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
Isotype Negative Control	Mouse IgG1 Isotype Control
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Use/Stability	Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

FTO (Fat mass-and obesity-associated gene) is the responsible gene for mouse ‘fused toes’ mutation. An association between FTO genotype and type 2 diabetes has been confirmed. The presence of the FTO rs9939609 A-allele was found to be positively correlated with other symptoms of the metabolic syndrome, including higher fasting insulin, glucose, triglycerides, and lower HDL-cholesterol.

Product References

A dynamic N6-methyladenosine methylome regulates intrinsic and acquired resistance to tyrosine kinase inhibitors: F. Yan, et al.; Cell Res. 28, 1062 (2018)
Gold Nanorods Exhibit Intrinsic Therapeutic Activity via Controlling N6-Methyladenosine-Based Epitranscriptomics in Acute Myeloid Leukemia: Y. Du, et al.; ACS Nano, 15, 17689 (2021)

Call	+41 61 926 6040
Fax	+41 61 926 6049