AdipoGen Life Sciences

anti-FTO (human), mAb (AG103)

CHF 300.00

In stock

AG-20A-0092-C05050 µgCHF 300.00

AG-20A-0092-C100100 µgCHF 390.00

Figure 1: Immunohistochemical staining of FTO with anti-FTO (human), mAb (AG103) (Prod. No. AG-20A-0092) in brain, hypothalamus and paraventricular nucleus (5~10 μg/ml).
This antibody has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalinfixed, paraffin-embedded human tissues. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain (40x).

Figure 2: Immunoprecipitation of recombinant human FTO proteins using anti-FTO (human), mAb (AG103) (Prod. No. AG-20A-0092) at different concentrations. The precipitated proteins were separated by SDS-PAGE, electroblotted, and visualized by Western blot with rabbit anti-mouse FTO pAb (Prod. No. AG-25A-0089).
1. hFTO (His-tagged) recombinant control 100ng (AG-40A-112).
2. hFTO (His-tagged) 5μg.
3. hFTO (His-tagged) 2.5μg.
4. hFTO (His-tagged) 1.25μg.
5. hFTO (His-tagged) 0.625μg.
6. hFTO (His-tagged) 0.312μg.
7. hFTO (His-tagged) 0.156μg.
8. hFTO (His-tagged) 0.078μg.

Figure 3: Western blot analysis of human FTO using anti-human FTO mAb (AG103) (Prod. No. AG-20A-0092) at 1:1,000 dilution:
1. hFTO (His-tagged) 100ng.
2. HEK293T 100μg.
3. HEK293E 100μg.
4. HepG2 100μg.
5. Hep3B 50μg.
6. VIT1 80μg.
7. THP1 74μg.
8. Molt4 96μg.
9. A549 48μg.

Specifications / Handling

More Information
Product Details
Synonyms	Fat Mass and Obesity-associated Protein; FATSO
Product Type	Monoclonal Antibody
Properties
Clone	AG103
Isotype	Mouse IgG2aκ
Immunogen/Antigen	Recombinant human FTO.
Application	ELISA: (direct and indirect: 1:2’000-1:5’000) Immunoprecipitation: (5μg/ml per sample in a final reaction volume of 500-1’000μl). Immunohistochemistry: (5-10μg/ml: paraffin sections) Western Blot: (1:2’000-1:5’000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.
Crossreactivity	Human
Specificity	Recognizes human FTO.
Purity Detail	Protein G-affinity purified.
Concentration	1mg/ml
Formulation	Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
Isotype Negative Control	Mouse IgG2a Isotype Control
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Use/Stability	Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

FTO (Fat mass-and obesity-associated gene) is the responsible gene for mouse ‘fused toes’ mutation. An association between FTO genotype and type 2 diabetes has been confirmed. The presence of the FTO rs9939609 A-allele was found to be positively correlated with other symptoms of the metabolic syndrome, including higher fasting insulin, glucose, triglycerides, and lower HDL-cholesterol.

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