AdipoGen Life Sciences

anti-LAG-3, mAb (blocking) (11E3)

CHF 470.00
In stock
AG-20B-0011-C100100 µgCHF 470.00
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Product Details
Synonyms Lymphocyte Activation Gene-3; FDC Protein; CD223
Product Type Monoclonal Antibody
Properties
Clone 11E3
Isotype Mouse IgG1
Source/Host Purified from concentrated hybridoma tissue culture supernatant.
Immunogen/Antigen Recombinant human LAG-3.
Application

ELISA: (capture)
Functional Application: Inhibition
Immunocytochemistry
Immunohistochemistry: (frozen sections)
Immunoprecipitation: (10μg/ml)
Western Blot: (5μg/ml)

Crossreactivity Human
Monkey
Specificity

Recognizes human and monkey LAG-3.

Purity ≥95% (SDS-PAGE)
Purity Detail Protein G-affinity purified.
Endotoxin Content <0.001EU/μg purified protein or <1EU/mg purified protein (LAL test; Lonza).
Concentration 0.5 mg/ml
Formulation Liquid. In PBS containing 0.02% sodium azide.
Isotype Negative Control

Mouse IgG1 Isotype Control

Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF
Description

Lymphocyte activation gene 3 (LAG-3; CD223) plays an important role in negatively regulating T cell proliferation, function and homeostasis. It is required for maximal natural and induced regulatory T cell (Treg) function. LAG-3 is closely related to the T cell co-receptor CD4 and binds to MHC class II molecules but with a significantly higher affinity than CD4.

Product References
  1. Cellular expression and tissue distribution of the human LAG-3-encoded protein, an MHC class II ligand: B. Huard, et al.; Immunogenetics 39, 213 (1994)
  2. Characterization of the major histocompatibility complex class II binding site on LAG-3 protein: B. Huard, et al.; PNAS 94, 5744 (1997)
  3. A soluble lymphocyte activation gene-3 (sLAG-3) protein as a prognostic factor in human breast cancer expressing estrogen or progesterone receptors: F. Triebel, et al.; Cancer Lett. 235, 147 (2006)
  4. Clonal regulatory T cells specific for a red blood cell autoantigen in human autoimmune hemolytic anemia: F.J. Ward, et al.; Blood 111, 680 (2008)
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