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AdipoGen Life Sciences
anti-LAG-3 (human), mAb (blocking) (17B4)
470
CHF
CHF 470.00
In stock
AG-20B-0012-C100100 µgCHF 470.00
| Product Details | |
|---|---|
| Synonyms | Lymphocyte Activation Gene-3; FDC Protein; CD223 |
| Product Type | Monoclonal Antibody |
| Properties | |
| Clone | 17B4 |
| Isotype | Mouse IgG1κ |
| Source/Host | Purified from concentrated hybridoma tissue culture supernatant. |
| Immunogen/Antigen | Synthetic peptide corresponding to 30 aa in the N-terminus of human LAG-3. |
| Application |
Flow Cytometry |
| Crossreactivity | Human |
| Specificity |
Recognizes human LAG-3. |
| Purity | ≥95% (SDS-PAGE) |
| Purity Detail | Protein G-affinity purified. |
| Endotoxin Content | <0.001EU/μg purified protein or <1EU/mg purified protein (LAL test; Lonza). |
| Concentration | 1mg/ml |
| Formulation | Liquid. In PBS containing 0.02% sodium azide. |
| Isotype Negative Control | |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
Lymphocyte activation gene 3 (LAG-3; CD223) plays an important role in negatively regulating T cell proliferation, function and homeostasis. It is required for maximal natural and induced regulatory T cell (Treg) function. LAG-3 is closely related to the T cell co-receptor CD4 and binds to MHC class II molecules but with a significantly higher affinity than CD4.
Product References
- Characterization of the lymphocyte activation gene 3-encoded protein. A new ligand for human leukocyte antigen class II antigens: E. Baixeras, et al.; J. Exp. Med. 176, 327 (1992)
- Cellular expression and tissue distribution of the human LAG-3-encoded protein, an MHC class II ligand: B. Huard, et al.; Immunogenetics 39, 213 (1994)
- Characterization of the major histocompatibility complex class II binding site on LAG-3 protein: B. Huard, et al.; PNAS 94, 5744 (1997)
- CD3/TCR complex-associated lymphocyte activation gene-3 molecules inhibit CD3/TCR signaling: S. Hannier, et al.; J. Immunol. 161, 4058 (1998)
- T Lymphocytes infiltrating various tumour types express the MHC class II ligand lymphocyte activation gene-3 (LAG-3): role of LAG-3/MHC class II interactions in cell-cell contacts: C.E. Demeure, et al.; Eur. J. Cancer 37, 1709 (2001)
- Immunological mechanisms elicited at the tumour site by lymphocyte activation gene-3 (LAG-3) versus IL-12: sharing a common Th1 anti-tumour immune pathway: E.D. Carlo, et al.; J. Pathol. 205, 82 (2005)
- The negative regulatory function of the lymphocyte-activation gene-3 co-receptor (CD223) on human T cells: L. Macon-Lemaitre and F. Triebel; Immunology 115, 170 (2005)
- A soluble lymphocyte activation gene-3 (sLAG-3) protein as a prognostic factor in human breast cancer expressing estrogen or progesterone receptors: F. Triebel, et al.; Cancer Lett. 235, 147 (2006)
- Human dendritic cells acquire a semimature phenotype and lymph node homing potential through interaction with CD4+CD25+ regulatory T cells: J. Bayry, et al.; J. Immunol. 178, 4184 (2007)
- Human lymphocyte activation gene-3 molecules expressed by activated T cells deliver costimulation signal for dendritic cell activation: C. Casati, et al.; J. Immunol. 180, 3782 (2008)
- Peripheral Tr1 and Foxp3+ Treg as Markers of Recurrent Malignancies in Patients with Hepato-Biliary Pancreatic Cancer: T. Ikemoto, et al.; Anticancer Res. 37, 5441 (2017)
- Blockade of LAG3 enhances responses of tumor-infiltrating T cells in mismatch repair-proficient liver metastases of colorectal cancer: G. Zhou, et al.; OncoImmunology 7, e1448332 (2018)
- Targeting LAG-3 and PD-1 to Enhance T Cell Activation by Antigen-Presenting Cells: F.S. Lichtenegger, et al.; Front. Immunol. 9, 385 (2018)
- Association Between Expression Level of PD1 by Tumor-Infiltrating CD8+ T Cells and Features of Hepatocellular Carcinoma: HD. Kim, et al.; Gastroenterology 155, 1936 (2018)
- A T-cell–engaging B7-H4/CD3-bispecific Fab-scFv Antibody Targets Human Breast Cancer: A. Iizuka, et al.; Clin. Cancer Res. 25, 2925 (2019)
- Efficient expansion of human granzyme B–expressing B cells with potent regulatory properties: M. Chesneau, et al.; J. Immunol. 205, 2391 (2020)
- Expansion of donor-unrestricted MAIT cells with enhanced cytolytic function suitable for TCR redirection: T. Parrot, et al.; JCI Insight 6, e140074 (2021)
- Single-cell profiling identifies impaired adaptive NK cells expanded after HCMV reactivation in haploidentical HSCT: E. Zaghi, et al.; JCI Insight 6, e146973 (2021)
- The multi-specific VH-based Humabody CB213 co-targets PD1 and LAG3 on T cells to promote anti-tumour activity: C.J. Edwards, et al.; Br. J. Cancer 126, 1168 (2022)
- Measurement of LAG-3 Expression Across Multiple Staining Platforms With the 17B4 Antibody Clone: J.B. Wojcik, et al.; Arch. Pathol. Lab. Med. 147, 1307 (2023) [IHC-FFPE]
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