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AdipoGen Life Sciences
anti-α-Tubulin (acetylated), mAb (TEU318)
390
CHF
CHF 390,00
In stock
AG-20B-0068-C100100 µgCHF 390.00
Figure 1: Western blot analysis of protein acetylation with anti-α-Tubulin (acetylated), mAb (TEU318) (Prod. No. AG-20B-0068).
Method: HEK-293T cells grown in standard culture conditions, transfected with plasmids expressing the tubulin acetyl transferase α-TAT1 (or MEC17; J.S. Akella, et al.; Nature 2010; T. Shida, et al.; PNAS 2010) and are lysed in Laemmli sample buffer. Brain tubulin was prepared following standard procedures (M. Castoldi & A.V. Popov; Protein Expr. Purif. 2003). Samples are run on a 10% SDS-PAGE and proteins are transferred to a nitrocellulose membrane and detected by standard immuno blot protocol using anti-α-Tubulin (acetylated), mAb (TEU318) (1:2’000) in TBS containing 0.1% Tween-20 for washing steps and 2.5% fat free milk for antibody incubation. In normal HEK cells, acetylated α-tubulin is detected, although faintly. Expression of α-TAT1 (or MEC17) acetyltransferase leads to enhanced acetylation of tubulin. Brain tubulin is highly acetylated and therefore is strongly detected.
Picture courtesy of Dr. Sudarshan Gadadhar & Dr. Carsten Janke, Curie Institute, Paris
Method: HEK-293T cells grown in standard culture conditions, transfected with plasmids expressing the tubulin acetyl transferase α-TAT1 (or MEC17; J.S. Akella, et al.; Nature 2010; T. Shida, et al.; PNAS 2010) and are lysed in Laemmli sample buffer. Brain tubulin was prepared following standard procedures (M. Castoldi & A.V. Popov; Protein Expr. Purif. 2003). Samples are run on a 10% SDS-PAGE and proteins are transferred to a nitrocellulose membrane and detected by standard immuno blot protocol using anti-α-Tubulin (acetylated), mAb (TEU318) (1:2’000) in TBS containing 0.1% Tween-20 for washing steps and 2.5% fat free milk for antibody incubation. In normal HEK cells, acetylated α-tubulin is detected, although faintly. Expression of α-TAT1 (or MEC17) acetyltransferase leads to enhanced acetylation of tubulin. Brain tubulin is highly acetylated and therefore is strongly detected.
Picture courtesy of Dr. Sudarshan Gadadhar & Dr. Carsten Janke, Curie Institute, Paris
Product Details | |
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Product Type | Monoclonal Antibody |
Properties | |
Clone | TEU318 |
Isotype | Mouse IgG1 |
Source/Host | Purified from concentrated hybridoma tissue culture supernatant. |
Immunogen/Antigen | Tubulin of the Ciliate Euplotes eluted from the 55kDa band of SDS gel. |
Application |
Western Blot: 1:2000 |
Crossreactivity | All |
Specificity |
Detects K40 acetylation of α-tubulin; signal specifically increased by modification with tubulin acetyl transferase α-TAT1. |
Purity | ≥95% (SDS-PAGE) |
Purity Detail | Protein G-affinity purified. |
Concentration | 1mg/ml |
Formulation | Liquid. In PBS containing 10% glycerol and 0.02% sodium azide. |
Isotype Negative Control | |
Shipping and Handling | |
Shipping | BLUE ICE |
Short Term Storage | +4°C |
Long Term Storage | -20°C |
Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
Documents | |
MSDS | Download PDF |
Product Specification Sheet | |
Datasheet | Download PDF |