anti-Polyglutamate chain (polyE), pAb (IN105)

CHF 440.00
In stock
AG-25B-0030-C05050 µgCHF 440.00
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Product Details
Product Type Polyclonal Antibody
Properties
Source/Host Rabbit
Immunogen/Antigen Polyglutamate peptide.
Application

Immunocytochemistry: (1:5'000)
Immunohistochemistry: (paraffin sections; 1:1000)
Western Blot: (1:2'000)

Crossreactivity All
Specificity

Recognizes C-terminally located linear alpha-glutamate chains of 4 and more glutamate residues.

Purity Detail Epitope-affinity purified.
Concentration 0.5mg/ml
Formulation Liquid. In PBS containing 0.02% sodium azide.
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF

Microtubules are key elements of the eukaryotic cytoskeleton that dynamically assemble from heterodimers of α- and β-tubulin. Two different mechanisms can generate microtubule diversity: the expression of different α- and β-tubulin genes, referred to as tubulin isotypes, and the generation of posttranslational modifications (PTMs) on α- and β-tubulin. Tubulin PTMs include the well-known acetylation or phosphorylation, and others that have so far mostly been found on tubulin, detyrosination/tyrosination, polyglutamylation and polyglycylation. These PTMs might have evolved to specifically regulate tubulin and microtubule functions. Polyglutamylation is a PTM that occurs when secondary glutamate side chains are formed on γ-carboxyl groups of glutamate residues in a protein. Enzymes catalyzing polyglutamylation belong to the TTL-like (TTLL; Tubulin tyrosine ligase-like) family of glutamylases. Deglutamylases, the enzymes that reverse polyglutamylation, were identified within a novel family of CCPs (cytosolic carboxypeptidase). Subtle differences in polyglutamylation can be seen on diverse microtubules in different cell types. The functions of these modifications remain to be studied. However, its wide distribution strengthens the idea that it could be involved in fine-tuning a range of microtubule functions. PolyE labels centrioles as they mature, such that two foci are present throughout the cell cycle.

Product References
  1. Tubulin polyglutamylation stimulates spastin-mediated microtubule severing: B. Lacroix, et al.; J. Cell Biol. 189, 945 (2010)
  2. Microtubule detyrosination guides chromosomes during mitosis: M. Barisic, et al.; Science 348, 6236 (2015) (Supplement)
  3. Zika virus causes supernumerary foci with centriolar proteins and impaired spindle positioning: B. Wolf, et al.; Open Bio 7, 160231 (2017)
  4. Alterations in the balance of tubulin glycylation and glutamylation inphotoreceptors leads to retinal degeneration: M. Bosch Grau, et al.; J. Cell. Sci. 130, 938 (2017)
  5. Spastin regulates VAMP7-containing vesicles trafficking in cortical neurons: C. Plaud, et al.; BBA - Molecular Basis of Disease 1863, 1666 (2017)
  6. iPSCs from a Hibernator Provide a Platform for Studying Cold Adaptation and Its Potential Medical Applications: J. Ou, et al.; Cell 173, 1 (2018)
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