AdipoGen Life Sciences

anti-CD3 (human), mAb (TR66)

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Product Details
Synonyms	CD3ε; CD3e; T Cell Surface Glycoprotein CD3 epsilon Chain; T Cell Surface Antigen T3/Leu-4 epsilon Chain; T3E
Product Type	Monoclonal Antibody
Properties
Clone	TR66
Isotype	Mouse IgG1
Source/Host	Purified from concentrated hybridoma tissue culture supernatant.
Immunogen/Antigen	Cloned human helper T cell line.
Application	Flow Cytometry Immunocytochemistry Immunoprecipitation Western Blot Functional Application: Activates T cells in vivo and in vitro, together with a costimulatory signal and cytokine support.
Crossreactivity	Human
Specificity	Recognizes the ε-chain of CD3.
Purity	≥95% (SDS-PAGE)
Purity Detail	Protein G-affinity purified.
Concentration	1mg/ml
Formulation	Liquid. In PBS containing 0.02% sodium azide.
Isotype Negative Control	Mouse IgG1 Isotype Control (preservative free)
Accession Number	https://www.uniprot.org/uniprotkb/P07766/entry
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Use/Stability	Stable for at least 1 year after receipt when stored at -20°C.
Documents
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

CD3 is a multi-subunit protein complex expressed on the surface of T lymphocytes as part of the T cell receptor (TCR) complex. It plays a central role in T cell activation by transmitting signals from the TCR upon antigen recognition. Because of its consistent expression across all mature T cells, CD3 serves as a key pan-T cell marker in immunology research. Anti-CD3 antibodies are widely used to study T cell biology and immune signaling. They are used to identify and characterize T cells or activate T cells in vitro by crosslinking the TCR/CD3 complex, enabling functional assays of proliferation and cytokine production. The versatile applications make anti-CD3 antibodies essential tools in immunology, cancer immunotherapy research and studies of autoimmune disease.

Product References

The use of hybrid hybridomas to target human cytotoxic T lymphocytes: A. Lanzavecchia & D. Scheidegger; Eur. J. Immunol. 17, 105 (1987)
Efficiency of T cell triggering by anti-CD3 monoclonal antibodies (mAb) with potential usefulness in bispecific mAb generation: N. Jacobs, et al.; Cancer Immunol. Immunother. 44, 257 (1997)
FLIP prevents apoptosis induced by death receptors but not by perforin/granzyme B, chemotherapeutic drugs, and gamma irradiation: T. Kataoka, et al.; J. Immunol. 161, 3936 (1998)
T lymphocyte costimulation mediated by reorganization of membrane microdomains: A. Viola, et al.; Science 283, 680 (1999)
Optimizing anti-CD3 affinity for effective T cell targeting against tumor cells: N. Bortoletto, et al; Eur. J. Immunol. 32, 3102 (2002)
T cell fitness determined by signal strength: A.V. Gett, et al.; Nat. Immunol. 4, 355 (2003)
Design, selection and optimization of an anti-TRAIL-R2/anti-CD3 bispecific antibody able to educate T cells to recognize and destroy cancer cells: A. Satta, et al.; MAbs 10, 1084 (2018)

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