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AdipoGen Life Sciences
anti-Adiponectin (human), pAb (Biotin)
390
CHF
CHF 390.00
In stock
AG-25A-0003B-C05050 µgCHF 390.00
Figure 1: Western blot analysis in human plasma using anti-Adiponectin (human), pAb (Biotin) (Prod. No. AG-25A-0003B) at 0.2μg/ml concentration.
Product Details | |
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Synonyms | ACRP30; AdipoQ; apM1; GBP28; Adipocyte Complement Related Protein of 30kDa |
Product Type | Polyclonal Antibody |
Properties | |
Source/Host | Rabbit |
Immunogen/Antigen | Recombinant human adiponectin. |
Label/Conjugates | Biotin |
Application |
ELISA: (direct and indirect; 1:5'000-1:10'000) Western Blot: (1:5'000-1:10'000 using ECL; suggested blocking and dilution buffer is TBST with 0.05% Tween 20 with 5% skim milk; incubate 1 hour at room temperature). Optimal conditions must be determined individually for each application. |
Crossreactivity | Human |
Specificity | Recognizes human adiponectin. Detects a band of ~30kDa by Western blot. |
Purity Detail | Protein A-affinity purified. |
Concentration | 1mg/ml |
Formulation | Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives. |
Shipping and Handling | |
Shipping | BLUE ICE |
Short Term Storage | +4°C |
Long Term Storage | -20°C |
Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
Use/Stability | Stable for at least 6 months after receipt when stored at -20°C. |
Documents | |
MSDS | Download PDF |
Product Specification Sheet | |
Datasheet | Download PDF |
Description
ACRP30 was identified as a novel adipocyte-specific synthesized and secreted protein with structural resemblance to complement factor C1q. Like adipsin, ACRP30 secretion is induced ~10-fold during adipocyte differentiation. Plasma levels are reduced in obese humans, and low levels are associated with insulin-resistance. Treatment of db/db mice with TZD increased ACRP30 levels.
Product References
- Plasma resistin concentrations measured by enzyme-linked immunosorbent assay using a newly developed monoclonal antibody are elevated in individuals with type 2 diabetes mellitus: B.S. Youn, et al.; J. Clin. Endocrinol. Metab. 89, 150 (2004)