AdipoGen Life Sciences

anti-RELM-β (human), pAb

CHF 190.00

In stock

AG-25A-0012-C100100 µgCHF 190.00

Figure 1: Immunohistochemical staining of RELM-β using anti-RELM-β (human), pAb (Prod. No. AG-25A-0012) in human small intestine tissue (5µg/ml).
This antibody has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin-fixed, paraffin-embedded human tissues. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.

Figure 2: Western blot analysis using anti-RELM-β (human), pAb (Prod. No. AG-25A-0012) at 1:5'000 dilution.
1: Human RELM-β.
2: Human Resistin.
3: Human Adiponectin.

Specifications / Handling

More Information
Product Details
Synonyms	HXCP2; Resistin-like Molecule-β; FIZZ2
Product Type	Polyclonal Antibody
Properties
Source/Host	Rabbit
Immunogen/Antigen	Recombinant human RELM-β.
Application	ELISA: (direct and indirect: 1:5’000-1:10’000) Immunohistochemistry: (5μg/ml) Western Blot: (1:5’000-1:10’000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application. Note: Tested on recombinant proteins and/or target-protein transfected cell lines in ELISA, Western Blot and/or FACS.
Crossreactivity	Human
Specificity	Recognizes human RELM-β. Detects a band of ~19kDa by Western blot. Does not detect human Resistin, mouse Resistin, mouse RELM-α, mouse RELM-β or mouse RELM-γ.
Purity Detail	Protein A-affinity purified.
Concentration	1mg/ml
Formulation	Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
Isotype Negative Control	Rabbit IgG
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Use/Stability	Stable for at least 6 months after receipt when stored at -20°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

RELM-β is a 19kDa disulfide-linked homodimeric protein expressed in the epithelium of the colon and small bowel. RELM-β has been suggested to play a regulatory role during inflammation and may also act to establish links among adipose tissue, the intestine and the liver. Interestingly, the molecular structure of RELM-β is highly homologous to that of the adipose-derived cytokine resistin.

Product References

Choosing the right antibody for resistin-like molecule (RELM/FIZZ) family members: C. Fan, et al.; Histochem. Cell Biol. 139, 605 (2013)

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