AdipoGen Life Sciences

anti-TRB-3 (human), pAb

CHF 190.00

In stock

AG-25A-0059-C100100 µgCHF 190.00

Figure 1: Immunohistochemical staining of of human tissue using anti-TRB3 (human), pAb (Prod. No. AG-25A-0059) at 1:2'000 dilution.
1: Immunoperoxidase staining (cytoplasmic) of formalin-fixed, paraffin-embedded human salivary glands (200x, brown colour).

Figure 2: Western blot analysis of human tissue lysate using anti-TRB3 (human), pAb (Prod. No. AG-25A-0059) at 1:2,000 dilution.
1. Human spleen tissue lysate (10μg).
2. Human liver tissue lysate (10μg)
3. Human colon tissue lysate (10μg)
4. Human Thpok (His-tagged) (negative control).

Specifications / Handling

More Information
Product Details
Synonyms	Tribbles Homolog 3; Neuronal Cell death-inducible Putative Kinase; SINK; p65-interacting Inhibitor of NF-κB
Product Type	Polyclonal Antibody
Properties
Source/Host	Rabbit
Immunogen/Antigen	Synthetic peptide corresponding to aa 112-125 (A¹¹²RLPPHKHVARPTE¹²⁵) of human TRB-3.
Application	ELISA: (direct and indirect: 1:2’000-1:5’000) Immunohistochemistry: (paraffin sections(1:500)) Western Blot: (1:2’000-1:5’000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application. Note: Tested on recombinant proteins and/or target-protein transfected cell lines in ELISA, Western Blot and/or FACS.
Crossreactivity	Human
Specificity	Recognizes human TRB-3. Detects a band of ~28kDa by Western blot (predicted 39kDa).
Purity Detail	Protein A-affinity purified.
Concentration	1mg/ml
Formulation	Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
Isotype Negative Control	Rabbit IgG
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Use/Stability	Stable for at least 6 months after receipt when stored at -20°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

Tribbles homolog 3 (TRB-3) is a pseudokinase, shown to act as a potent negative regulator of PPARγ, a master regulator of adipocyte differentiation, and to control adipogenesis. It disrupts insulin signaling by binding directly to Akt kinases and blocking their activation. It interacts also with the NF-κB transactivator p65 RELA. May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells.

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