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AdipoGen Life Sciences
anti-IL-37 (human), pAb
320
CHF
CHF 320.00
In stock
AG-25A-0111-C100100 µgCHF 320.00
Figure 1: Western blot analysis using anti-IL-37 (human), pAb (Prod. No. AG-25A-0111) at 1:2.000 dilution:
1. Recombinant human IL-37-His (50ng)
2. Human IL-37-FLAG transfected HEK293 cell lysate(100µg)
3. Human IL-37-tag free transfected HEK293 cell lysate(100µg)
4. Empty vector transfected HEK293 cell lysate(100µg)
5. A unrelated protein-His (50ng)
1. Recombinant human IL-37-His (50ng)
2. Human IL-37-FLAG transfected HEK293 cell lysate(100µg)
3. Human IL-37-tag free transfected HEK293 cell lysate(100µg)
4. Empty vector transfected HEK293 cell lysate(100µg)
5. A unrelated protein-His (50ng)
Figure 2: Immunoprecipitation of human IL-37 using anti-IL-37 (Prod. No. AG-25A-0111) at 1:500 dilutions:
1. Recombinant human IL-37-His (1µg)
2. Human IL-37-FLAG transfected HEK293 cell lysate(500µg)
3. Human IL-37-tag free transfected HEK293 cell lysate(500µg)
4. Empty vector transfected HEK293 cell lysate(500µg)
5. A unrelated protein-His (1µg)
1. Recombinant human IL-37-His (1µg)
2. Human IL-37-FLAG transfected HEK293 cell lysate(500µg)
3. Human IL-37-tag free transfected HEK293 cell lysate(500µg)
4. Empty vector transfected HEK293 cell lysate(500µg)
5. A unrelated protein-His (1µg)
Figure 3: Immunohistochemical staining of IL-37 in human rheumatoid arthritis tissue.
Method: Immunohistochemical analysis of a paraffin-embedded rheumatoid arthritis tissue, showing endothelial cells and plasma cells (mature B cells) stained by anti-IL-37 (Prod. No. AG-25A-0111) at dilution 1:50 (under X200 lens, Brown color).
Method: Immunohistochemical analysis of a paraffin-embedded rheumatoid arthritis tissue, showing endothelial cells and plasma cells (mature B cells) stained by anti-IL-37 (Prod. No. AG-25A-0111) at dilution 1:50 (under X200 lens, Brown color).
Figure 4: Immunohistochemical staining of IL-37 in human kidney (A1), human colon (B1), human skin (C) and human stomach (D) using anti-IL-37 (Prod. No. AG-25A-0111). Human kidney (A2) and human colon (B2) are stained with negative control anti-rabbit IgG.
| Product Details | |
|---|---|
| Synonyms | Interleukin-1 Family Member 7; IL-1F7; FIL1 zeta; IL-1X; Interleukin-1 Homolog 4; IL-1H4; Interleukin-1 zeta; IL-1 zeta; IL-1RP1; IL1F7; FIL1Z, IL1H4, IL1RP1 |
| Product Type | Polyclonal Antibody |
| Properties | |
| Source/Host | Rabbit |
| Immunogen/Antigen | Recombinant human IL-37. |
| Application |
ELISA: (direct and indirect: 1:2’000-1:5’000) Note: Tested on recombinant proteins and/or target-protein transfected cell lines in ELISA, Western Blot and/or FACS. |
| Crossreactivity | Human |
| Specificity |
Recognizes human IL-37. Detects a band of ~30kDa by Western blot. |
| Purity Detail | Protein A-affinity purified. |
| Concentration | 1 mg/ml |
| Formulation | Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives. |
| Isotype Negative Control | |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 6 months after receipt when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
IL-37 (IL-1F7; IL-1H4) is an IL-1 family member that is expressed only in certain types of human organs and cells such as heart, thymus, testis, kidney, mononuclear cells (PBMCs) and dendritic cells. IL-37 is an inhibitor of innate immunity with anti-inflammatory properties. It binds to the interleukin-18 receptor (IL-18R) and its co-receptor SIGIRR. IL-37 is secreted as a full-length and as a processed form starting from amino acid Val46. It plays an important role as a link between innate and adaptive immunity and acts as an inhibitor of autoimmune diseases and tumor growth. IL-37 also inhibited Lipopolysaccharide (LPS)-induced immunological reaction and LPS-induced osteoclast formation and bone resorption.
Product References
- Early transcriptional changes after UVB treatment in atopic dermatitis include inverse regulation of IL-36γ and IL-37: AH. Lossius, et al.; Exp. Dermatol. 30, 249 (2021)
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