anti-Angiopoietin-2, mAb (rec.) (blocking) (Angy-2-1) (preservative free)

CHF 440.00
In stock
AG-27B-0016PF-C100100 µgCHF 440.00
AG-27B-0016PF-C500500 µgCHF 1'200.00
AG-27B-0016PF-M0011 mgCHF 2'000.00
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Product Details
Synonyms Ang-2; Ang2; Angpt2; Agpt2
Product Type Recombinant Antibody
Properties
Clone Angy-2-1
Isotype Mouse IgG2bλ.
Source/Host Produced without the use of animals. Purified from CHO cell culture supernatant.
Immunogen/Antigen Recombinant human angiopoietin-2.
Label/Conjugates Preservative Free
Application

ELISA
Functional: Inhibits the binding of mouse angiopoietin-2 to mouse Tie-2. ND50* = 50-60ng/ml (for 10ng/ml of mouse Angiopoietin-2) 
Inhibits the binding of human angiopoietin-2 to human Tie-2. ND50* = 8-12ng/ml (for 10ng/ml of human Angiopoietin-2) 
*ND50: 50% neutralizing dose of antibody for a given concentration of ligand (here Angiopoietin-2).
ICC: 1:100

Crossreactivity Human
Mouse
Specificity

Recognizes human and mouse angiopoietin-2. Does not detect human angiopoietin-1.

Purity ≥95% (SDS-PAGE)
Purity Detail Protein A-affinity purified.
Concentration 1mg/ml
Formulation Liquid. In PBS.
Isotype Negative Control

Mouse IgG2b Isotype Control (preservative free

Other Product Data

anti-Angiopoietin-2, mAb (rec.) (blocking) (Angy-2-1) (preservative free) is composed of human variable regions (VH and VL) (λ-chain) of immunoglobulin fused to the mouse lgG2b Fc domain.

Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF

Angiopoietin-1 (Ang-1) and Angiopoietin-2 (Ang-2) are closely related secreted ligands which bind with similar affinity to Tie-2. Tie-2 and angiopoietins have been shown to play critical roles in embryogenic angiogenesis and in maintaining the integrity of the adult vasculature. Ang-1 activates Tie-2 signaling on endothelial cells to promote chemotaxis, cell survival, cell sprouting, vessel growth and stabilization. Ang-2 has been identified as a secreted protein ligand of Tie-2 and has alternatively been reported to be an antagonist for Ang-1 induced Tie-2 signaling as well as an agonist for Tie-2 signaling, depending on the cell context.

anti-Angiopoietin-2, mAb (rec.) (blocking) (Angy-2-1) (preservative free) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.

Product References
  1. Elevated angiopoietin-2 level in patients with continuous-flow left ventricular assist devices leads to altered angiogenesis and is associated with higher nonsurgical bleeding: C.E. Tabit, et al.; Circulation 134, 141 (2016)
  2. Angiopoietin 2 induces astrocyte apoptosis via αvβ5-integrin signaling in diabetic retinopathy: J.H. Yun, et al.; Cell Death Dis. 7, e2101 (2016)
  3. Angiopoietin-2 in white adipose tissue improves metabolic homeostasis through enhanced angiogenesis: Y.A. An, et al.; eLife 6, e24071 (2017)
  4. Hyperinsulinemia promotes endothelial inflammation via increased expression and release of Angiopoietin-2: S. Chandel, et al.; Atherosclerosis 307, 1 (2020)
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