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AdipoGen Life Sciences
anti-IgG (human), mAb (rec.) Multiclonal (HRP)
Product Details | |
---|---|
Synonyms | Immunoglobulin Heavy Constant Gamma |
Product Type | Recombinant Antibody |
Properties | |
Isotype | Mouse IgG2a |
Source/Host | Produced without the use of animals. Purified from HEK 293 cell culture supernatant. |
Immunogen/Antigen | Human intravenous immunoglobulin (IVIG). |
Label/Conjugates | Horseradish peroxidase |
Application |
ELISA |
Crossreactivity | Human |
Specificity |
Recognizes different epitopes on all four different subclasses of human IgG. |
Purity | ≥95% (SDS-PAGE) |
Purity Detail | Protein A purified from animal component-free supernatant. |
Concentration | 1 mg/ml |
Formulation | Liquid. In PBS. |
Isotype Negative Control | |
Accession Number | P01857-P01859-P01860-P01861 |
Declaration | Manufactured by Abcalis |
Shipping and Handling | |
Shipping | BLUE ICE |
Short Term Storage | +4°C |
Long Term Storage | +4°C |
Handling Advice | Please handle under sterile conditions to avoid contamination. |
Use/Stability | Stable for at least 3 months after receipt when stored at +4°C. |
Documents | |
MSDS | Download PDF |
Product Specification Sheet | |
Datasheet | Download PDF |
Multiclonals are recombinant secondary antibodies that combine the best of polyclonal antisera and hybridoma monoclonal antibodies, while eliminating their disadvantages, plus they add the quality of recombinant reagents. Multiclonal antibodies consist of carefully adjusted mixtures recognizing different epitopes on all four different subclasses of human IgG. Their respective epitope binding sites do not compete with each other, therefore amplifying signal strengths. Multiclonals can contain up to 17 different individually tested monoclonal recombinant antibodies. This provides the typical advantage of polyepitope recognition which is key to the broad application profile of polyclonal antisera, but eliminates their disadvantages (limited batch sizes and batch-to-batch variations, no long-term reproducibility, undefined composition, unknown constituents). Multiclonals are completely sequence defined, implying their unlimited long-term availability and always identical test results. Their composition of individually characterised antibodies minimizes cross-reactivity with other targets, since they do not contain non-target directed IgG like all animal derived polyclonals do. This allows for a much lower unspecific binding reactivity in many assays compared to animal based products. This purified antibody panel is conjugated to horseradish peroxidase (HRP) and ideal for diagnostic and research applications.
- Multiklonale Antikoerper als Ersatz fuer Zweitantikoerper aus Seren: E.V. Wenzel, et al.; BIOspektrum 26, 416 (2020) (German)
- Animal-free alternatives and the antibody iceberg: A. Gray, et al.; Nat. Biotechnol. 38, 1234 (2020)