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AdipoGen Life Sciences
Netrin-1 (human) (rec.)
300
CHF
CHF 300.00
In stock
AG-40B-0040-C01010 µgCHF 300.00
AG-40B-0040-30103 x 10 µgCHF 600.00
AG-40B-0040-C100100 µgCHF 1'320.00
| Product Details | |
|---|---|
| Synonyms | NTN1 |
| Product Type | Protein |
| Properties | |
| Source/Host | HEK 293 cells |
| Sequence | Human netrin-1 (aa 25-604) is fused at the C-terminus to a FLAG®-tag. |
| Crossreactivity |
Human Mouse Rat |
| Specificity | Binds to human, mouse and rat UNC5B. |
| Biological Activity | Induces pluripotent stem cell (iPS) formation and improves reprogramming efficiency of human and mouse somatic cells by inhibiting apoptosis mediated by the receptors DCC or UNC5B (at 150ng /ml). Induces axon outgrowth. |
| MW | ~80kDa (SDS-PAGE) |
| Purity | ≥95% (SDS-PAGE) |
| Endotoxin Content | <0.01EU/μg purified protein (LAL test; Lonza). |
| Concentration |
10µg vial: 0.1mg/ml after reconstitution. 100µg vial: 1mg/ml after reconstitution. |
| Reconstitution | Reconstitute with 100μl sterile distilled water. |
| Formulation | Lyophilized. Contains PBS plus protein stabilizer. No BSA included. The protein stabilizer shows no interference with protein activity. |
| Other Product Data | UniProt link O95631: Netrin-1 (human) |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1% BSA should be used for further dilutions. |
| Use/Stability |
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
Netrin-1 controls guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. It also serve as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Netrin-1 is also Involved in tumorigenesis by regulating apoptosis. Netrin-1 promotes atherosclerosis by retaining macrophages in the artery wall. Netrin-1 also governs induced pluripotent stem cell (iPS) formation and improves reprogramming efficiency of human and mouse somatic cells by limiting apoptosis mediated by Netrin-1 receptors DCC or UNC5b.
Product References
- Netrin-1 regulates somatic cell reprogramming and pluripotency maintenance: D. Ozmadenci, et al.; Nat. Commun. 6, ID7398 (2015)
- Targeting netrin‐1/DCC interaction in diffuse large B‐cell and mantle cell lymphomas: L. Broutier, et al.; EMBO Mol. Med. 8, 96 (2016)
- Inhibition of DNA methylation promotes breast tumor sensitivity to netrin‐1 interference: M. Grandin, et al.; EMBO Mol. Med. 8, 863 (2016)
- Structural Decoding of the Netrin-1/UNC5 Interaction and its Therapeutical Implications in Cancers: M. Grandin, et al.; Cancer Cell 29, 173 (2016)
- Ultrasound molecular imaging as a non-invasive companion diagnostic for netrin-1 interference therapy in breast cancer: J. Wischhusen, et al.; Theranostics 8, 5126 (2018)
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