Cookie Policy: This site uses cookies to improve your experience. You can find out more about our use of cookies in our Privacy Policy. By continuing to browse this site you agree to our use of cookies.
AdipoGen Life Sciences
Netrin-1 (human):Fc (human) (rec.)
350
CHF
CHF 350.00
In stock
AG-40B-0075-C01010 µgCHF 350.00
AG-40B-0075-30103 x 10 µgCHF 695.00
AG-40B-0075-C100100 µgCHF 1'560.00
Figure 1: Netrin-1 (human):Fc (human) (rec.) (Prod. No. AG-40B-0075) triggers a DCC-dependent phosphorylation of ERK1/2.
Method: HEK 293 cells (Control) or HEK 293 expressing the netrin-1 receptor DCC were incubated with netrin-1 (human):Fc (human) (rec.) (5 nM) for the indicated time points (shown in minutes). Antibodies against DCC, total ERK1/2 or phospho-ERK1/2 were used and visualized with a chemiluminescence detection system. Picture courtesy of Nicolas Rama, Prof. Patrick Mehlen lab, Centre Léon Bérard, Lyon
Method: HEK 293 cells (Control) or HEK 293 expressing the netrin-1 receptor DCC were incubated with netrin-1 (human):Fc (human) (rec.) (5 nM) for the indicated time points (shown in minutes). Antibodies against DCC, total ERK1/2 or phospho-ERK1/2 were used and visualized with a chemiluminescence detection system. Picture courtesy of Nicolas Rama, Prof. Patrick Mehlen lab, Centre Léon Bérard, Lyon
Figure 2: Netrin-1 (human):Fc (human) (rec.) (Prod. No. AG-40B-0075) induces outgrowth of the commisural axon.
Method: Dorsal spinal cords were dissected out from E13 rat embryos and cultured in collagen matrix in the presence or absence of netrin-1 (250ng/ml). Axons were then stained with an anti-β-tubulin antibody. Picture courtesy of Dr. Véronique Corset, Prof. Patrick Mehlen lab, Centre Léon Bérard, Lyon
Method: Dorsal spinal cords were dissected out from E13 rat embryos and cultured in collagen matrix in the presence or absence of netrin-1 (250ng/ml). Axons were then stained with an anti-β-tubulin antibody. Picture courtesy of Dr. Véronique Corset, Prof. Patrick Mehlen lab, Centre Léon Bérard, Lyon
| Product Details | |
|---|---|
| Synonyms | NTN1 |
| Product Type | Protein |
| Properties | |
| Source/Host | HEK 293 cells |
| Sequence |
Human netrin-1 (aa 25-604) is fused at the C-terminus to the Fc portion of human IgG1. |
| Crossreactivity |
Human Mouse Rat |
| Specificity |
Binds to human, mouse and rat UNC5B. |
| Biological Activity |
Induces pluripotent stem cell (iPS) formation and improves reprogramming efficiency of human and mouse somatic cells by inhibiting apoptosis mediated by the receptors DCC or UNC5B (at 150ng /ml). Induces axon outgrowth. |
| MW | ~90kDa (SDS-PAGE) |
| Purity | ≥95% (SDS-PAGE) |
| Endotoxin Content | <0.1EU/μg purified protein (LAL test; Lonza). |
| Concentration | 0.1mg/ml after reconstitution. |
| Reconstitution | Reconstitute with 100μl sterile distilled water. |
| Formulation | Lyophilized. Contains PBS plus protein stabilizer. No BSA included. The protein stabilizer shows no interference with protein activity. |
| Protein Negative Control | |
| Other Product Data |
UniProt link O95631: Netrin-1 (human) |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1% BSA should be used for further dilutions. |
| Use/Stability |
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
Netrin-1 controls guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. It also serve as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Netrin-1 is also Involved in tumorigenesis by regulating apoptosis. Netrin-1 promotes atherosclerosis by retaining macrophages in the artery wall. Netrin-1 also governs induced pluripotent stem cell (iPS) formation and improves reprogramming efficiency of human and mouse somatic cells by limiting apoptosis mediated by Netrin-1 receptors DCC or UNC5b.
Product References
- DCC constrains tumour progression via its dependence receptor activity: M. Castets, et al.; Nature 482, 534 (2011)
- The amyloid precursor protein regulates netrin-1 mediated commissural axon outgrowth: N. Rama, et al.; J. Biol. Chem. 287, 30014 (2012)
- Signaling switch of the axon guidance receptor Robo3 during vertebrate evolution: P. Zelina, et al.; Neuron 84, 1258 (2014)
- Netrin-1 regulates somatic cell reprogramming and pluripotency maintenance: D. Ozmadenci, et al.; Nat. Commun. 6, ID7398 (2015)
- Development of Targeted Microbubbles for Ultrasound Molecular Imaging of Breast Cancer: J. Wischhusen, et al.; RITS 150 (2015)
- Structural Decoding of the Netrin-1/UNC5 Interaction and its Therapeutical Implications in Cancers: M. Grandin, et al.; Cancer Cell 29, 173 (2016)
Related Products
