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Figure 1: SARS-CoV-2 Spike Protein S1 (RBD):Fc (human) (rec.) (B.1.351 Variant, Beta) (Prod. No. AG-40B-0203) binds to the receptor ACE2 (human) (rec.) slightly stronger than SARS-CoV-2 Spike Protein S1 (RBD):Fc (human) (rec.) (WT) (Prod. No. AG-40B-0194). Method: ACE2 (human) (rec.) (Prod. No. AG-40B-0192) is coated on an ELISA plate at 1μg/ml overnight at 4°C. Spike (SARS-CoV-2):Fc (human) (RBD) (rec.) (wt and variant B.1.351, respectively) or a control-Fc is added (starting at a concentration of 1000ng/ml with a twofold serial dilution) during one hour at RT and the interaction is then detected using an anti-human IgG (HRP).
2019-nCoV Spike Protein S1 (RBD); Spike Receptor Binding Domain Variant South Africa; 501Y.V2
Product Type
Protein
Properties
Source/Host
HEK 293 cells
Sequence
Receptor-binding domain (RBD) of SARS-CoV-2 Spike protein S1 (aa 319-541) containing the mutations K417N, E484K, N501Y is fused to the N-terminus of the Fc region of human IgG1.
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. For maximum product recovery after thawing, centrifuge the vial before opening the cap.
Use/Stability
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
SARS-CoV-2 shares 79.5% sequence identity with SARS-CoV and is 96.2% identical at the genome level to the bat coronavirus BatCoV RaTG133, suggesting it had originated in bats. The coronaviral genome encodes four major structural proteins: the Spike (S) protein, Nucleocapsid (N) protein, Membrane/Matrix (M) protein and the Envelope (E) protein. The SARS Envelope (E) protein contains a short palindromic transmembrane helical hairpin that seems to deform lipid bilayers, which may explain its role in viral budding and virion envelope morphogenesis. The SARS Membrane/Matrix (M) protein is one of the major structural viral proteins. It is an integral membrane protein involved in the budding of the viral particles and interacts with SARS Spike (S) protein and the Nucleocapsid (N) protein. The N protein contains two domains, both of them bind the virus RNA genome via different mechanisms.
The CoV Spike (S) protein assembles as trimer and plays the most important role in viral attachment, fusion and entry. It is composed of a short intracellular tail, a transmembrane anchor and a large ectodomain that consists of a receptor binding S1 subunit (RBD domain) and a membrane-fusing S2 subunit. The S1 subunit contains a receptor binding domain (RBD), which binds to the cell surface receptor angiotensin-converting enzyme 2 (ACE2) present at the surface of epithelial cells. Recently, a new variant of SARS-CoV-2, called B.1.351 (Beta), was detected in South Africa. This variant carries three mutations in the RBD at the positions 417, 484 and 501 (K417N, E484K, N501Y) and is associated with a higher viral load, which may suggest potential for increased transmissibility.
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