AdipoGen Life Sciences

IL-2 Superkine (monomeric):Fc (LALA-PG)-KIH (human) (rec.)

CHF 440.00
In stock
AG-40B-0262-C05050 µgCHF 440.00
NEW
More Information
Product Details
Synonyms InVivoKine™; IL-2 Superkine (monomeric):Fc (LALA-PG) Knobs-into-Holes (human) (rec.); Interleukin-2; T Cell Growth Factor; TCGF; Aldesleukin; Super-2
Product Type Protein
Properties
Source/Host HEK 293 cells. Produced using animal component-free medium.
Sequence

Human IL-2 (aa 21-153) (mutant H9 containing the mutations L80F / R81D / L85V / I 86V / I92F) is fused at the C-terminus to the Fc portion of human IgG1 (LALA-PG) (Knobs-into-Holes technology) (see reference: J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996)).

Crossreactivity Human
Mouse
Application

Triggers far greater antitumor responses than native IL-2 in vivo but with lower toxicity.

Specificity

Binds to human and mouse IL-2R (without CD25 requirement).

Biological Activity

Increases proliferation of CTLL-2 mouse cytotoxic T cells with an ED50 of 5-20 pM (~0.43 to 1.7 ng/ml).

MW ~45kDa and 28kDa (SDS-PAGE)
Purity ≥95% (SDS-PAGE)
Endotoxin Content <0.01EU/μg protein (LAL test).
Concentration After reconstitution: 1mg/ml
Reconstitution Reconstitute with 50μl endotoxin-free water.
Accession Number P60568
Formulation Lyophilized. Contains PBS.
Protein Negative Control

Fc (LALA-PG)-KIH (human) IgG1 Control (rec.) (Prod. No. AG-35B-0018)

Other Product Data

Uniprot link P60568: IL-2 (human)
AfCS Signaling Gateway link A006222: IL-2 (mouse)

Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Centrifuge lyophilized vial before opening and reconstitution.
PBS containing at least 0.1% BSA should be used for further dilutions.
Use/Stability Stable for at least 6 months after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF
Description

Interleukin-2 (IL-2) is a 133 amino acid glycoprotein with one intramolecular disulfide bond and variable glycosylation. It is secreted by activated T cells and induces proliferation and maturation of activated T cells, natural killer cells and lymphokine activated killer cells. IL-2 also stimulates proliferation of antibody-producing B cells, activates neutrophils and induces mononuclear cells to secrete IFN-γ and TNF-α and -β. Moreover, studies have shown that IL-2 is required for activation-induced apoptosis, an important homeostatic mechanism in the immune system, which is involved in the maintenance of peripheral tolerance to self-antigens.

IL-2 promotes T cell proliferation and particularly naive T cells. IL-2 signaling on activated T cells is effected through a quaternary high-affinity receptor complex consisting of IL-2, IL-2Rα (CD25), IL-2Rβ and IL-2Rγ. Naive T cells are relatively insensitive to IL-2 as they only express small amounts of IL-2Rβ and IL-2Rγ. They only acquire sensitivity after CD25 expression, which captures the cytokine and presents it to the IL-2Rβ and IL-2Rγ receptors. IL-2 Superkine (Fc) is an artificial variant of IL-2 called H9, containing mutations at positions L80F / R81D / L85V / I 86V / I92F. These mutations are located in the molecule's core that acts to stabilize the structure and to give it a receptor-binding conformation mimicking native IL-2 bound to CD25. These mutations effectively eliminate the functional requirement of IL-2 for CD25 expression and elicit proliferation of T cells. Compared to IL-2, the IL-2 superkine induces superior expansion of cytotoxic T cells, leading to improved anti-tumor responses in vivo, and elicits proportionally less toxicity by lowering the expansion of T-regulatory cells and reducing pulmonary oedema.

The protein IL-2 Superkine (monomeric):Fc (LALA-PG)-KIH (human) (rec.) is produced by using two different vectors, one encoding for the IL-2 Superkine:Fc (LALA-PG) Knobs sequence (synthesizing a protein of 45kDa) and one encoding for the Fc Holes sequence (synthesizing a protein of 28kDa). Both vectors transfected into HEK293 cells produce both Fc molecules (Knobs-into-Holes technology; J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996)) required for dimerization of the Fc moieties and for secretion of the final protein IL-2 Superkine (monomeric):Fc (LALA-PG)-KIH (human) (rec.). The LALA-PG mutations inhibit binding to FcγRs and C1q while FcRn binding and Fc stability remain unaffected.

InVivoKines™ are a new generation of recombinant fusion proteins for immunotherapeutic, preclinical and translational in vivo research, developed and manufactured by AdipoGen Life Sciences.

© 2017 Adipogen Life Sciences. Pictures: © 2012 Martin Oeggerli. All Rights Reserved.