AdipoGen Life Sciences

GLP-1R (human) (rec.) (His)

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Figure 1: GLP-1R (human) (rec.) (His) (Prod. No. AG-40B-0279) binds to its ligands Tirzepatide and Semaglutide. Method: The ligands Tirzepatide (Prod. No. AG-CP3-0043) and Semaglutide (Prod. No. AG-CP3-0040) are coated on an ELISA plate at 200nM overnight at room temperature. GLP-1R (human) (rec.) (His) (Prod. No. AG-40B-0279) is added (starting at 1000ng/ml with a two-fold serial dilution) during one hour and then detected using an anti-His (HRP) antibody. EC50 of the binding is about 5 ng/ml for both ligands.

Specifications / Handling

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Product Details
Synonyms	Glucagon-like Peptide 1 Receptor; GLP1R
Product Type	Protein
Properties
Source/Host	HEK 293 cells. Produced using animal component-free medium.
Sequence	Extracellular domain of human GLP-1R (aa 24-139) is fused at the C-terminus to the His Tag.
Crossreactivity	Bovine Human Mouse Rat
Application	Can be used to block GLP-1 in vitro or for high-throughput screening (HTS) assays (binding of GLP-1 analogs). Potential use for screening of new GLP-1R agonists.
Specificity	Binds to GLP-1, Semaglutide and Tirzepatide. Cross-reacts with human, mouse, rat and bovine GLP-1.
MW	~35-40kDa (SDS-PAGE)
Purity	≥95% (SDS-PAGE)
Endotoxin Content	<0.01 EU/μg protein (LAL test).
Concentration	After reconstitution: 1mg/ml
Reconstitution	Reconstitute with 50μl endotoxin-free water.
Accession Number	P43220
Formulation	Lyophilized. Contains PBS.
Other Product Data	Uniprot link P43220: GLP-1R (human)
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1% BSA should be used for further dilutions.
Use/Stability	Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

Glucagon-like peptide 1 (GLP-1) is a 30-amino acid Incretin peptide hormone produced in the intestine by differential processing of proglucagon. Incretin hormones are gut peptides that are secreted after nutrient intake and stimulate insulin secretion together with hyperglycemia. GIP (glucose-dependent insulinotropic polypeptide) and GLP-1 (glucagon-like peptide-1) are the known incretin hormones from the upper (GIP, K cells) and lower (GLP-1, L cells) gut. GLP-1 receptors are abundant in the hypothalamus which contains projections to the hunger centers. GLP-1 decreases calorie intake, boosts feelings of satiety and encourages weight loss. GLP-1 and GIP hormones are used as drugs for weight loss in obese patients and also to lower blood sugar levels.

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