AdipoGen Life Sciences

IL-9 (human) (monomer):Fc (silent) InVivoKine™

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AG-40B-0289-C05050 µgCHF 440.00

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Product Details
Synonyms	Interleukin-9 (human) (monomer):Fc (LALA-PG) KIH (human) (rec.); Cytokine P40; T-cell growth factor P40
Product Type	Protein
Properties
Source/Host	HEK 293 cells. Produced using animal component-free medium.
Sequence	Human IL-9 (aa 19-144) is fused at the C-terminus to the Fc portion of human IgG1 (LALA-PG) (Knobs-into-Holes technology) (see reference: J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996)).
Crossreactivity	Mouse
Specificity	The Fc contains the mutations LALA-PG that abolish the interaction between the Fc and FcγRs and therefore Fc undesirable effects. The Fc (LALA-PG) is named Fc (silent).
MW	~55kDa and 28kDa (SDS-PAGE)
Purity	≥95% (SDS-PAGE)
Endotoxin Content	<0.03EU/μg protein (LAL test).
Concentration	After reconstitution: 1mg/ml
Reconstitution	Reconstitute with 50μl endotoxin-free water.
Accession Number	P15248
Formulation	Lyophilized. Contains PBS.
Protein Negative Control	Fc (silent) InVivoKine™ (human) IgG1 Control (Prod. No. AG-35B-0018)
Other Product Data	Uniprot link P15248: IL-9 (human)
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1% BSA should be used for further dilutions.
Use/Stability	Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

The common cytokine receptor γ chain (γ c) family of cytokines includes interleukin-2 (IL-2), IL-4, IL-7, IL-9, IL-15, and IL-21. Each of these cytokines shares γ c as a receptor component. This set of cytokines exhibits pleiotropic functions on both the innate and adaptive immune systems, contributing to the development of T, B, natural killer (NK), and innate lymphoid (ILCs) cells. IL-9 is a late T cell growth factor as well as a mast cell growth factor. IL-9 is produced by TH9 cells, and also by ILC2 cells, mast cells, and NK T cells. IL-9 can regulate the function of T cells, B cells, mast cells and airway epithelium cells by activating the STAT1, STAT3 and STAT5 signaling pathways.

Recently, IL-9 /IL-9R axis has been shown to be an efficient tool to fight tumors. Indeed, compared with receptors for other common γc cytokines, IL9R is expressed at low levels and in very few cells. Exploiting the paucity of wild-type IL-9R expression and the safety of high doses of IL-9, T cells engineered with wild-type IL-9R exhibited superior tissue infiltration, stemness, and anti- tumor activity

The IL-9 (human) (monomer):Fc (silent) InVivoKine™ is produced by using two different vectors, one encoding for the IL-9 (human):Fc (LALA-PG) Knobs sequence (synthesizing a protein of 55 kDa) and one encoding for the Fc (LALA-PG) Holes sequence (synthesizing a protein of 28kDa). Both vectors transfected into HEK 293 cells produce both Fc molecules (Knobs-into-Holes technology; J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996) required for dimerization of the Fc moieties and for secretion of the final protein IL-9 (human) (monomer):Fc (silent) InVivoKine™. The LALA-PG mutations inhibit binding to FcγRs and C1q, while FcRn binding and Fc stability remain unaffected.

InVivoKines™ are a new generation of recombinant fusion proteins for immunotherapeutic, preclinical, and translational in vivo research, developed and manufactured by AdipoGen Life Sciences.

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