PD-L1 (human) ELISA Kit

CHF 620.00
In stock
AG-45B-0016-KI0196 wellsCHF 620.00
NEW
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Product Details
Synonyms Programmed Cell Death 1 Ligand Protein 1; PDCD1 Ligand 1; B7-H1; PDL1; CD274
Product Type Kit
Properties
Application Set Quantitative ELISA
Specificity

Detects soluble human PD-L1 (sPD-L1) in serum, plasma and cell culture supernatant.

Crossreactivity Human
Quantity

1 x 96 wells

Sensitivity 0.8pg/ml
Range 2.34 to 150pg/ml
Sample Type Cell Culture Supernatant
Plasma
Serum
Assay Type Sandwich
Detection Type Colorimetric
Other Product Data

UniProt link Q9NZQ7: PD-L1 (human)

Declaration In collaboration with Suzhou Bright Scistar Biotechnology
Accession Number Q9NZQ7
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage +4°C
Handling Advice After standard reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Plate and reagents should reach room temperature before use.
Use/Stability 12 months after the day of manufacturing. See expiry date on ELISA Kit box.
Documents
Manual Download PDF
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF

PD-L1 (also known as Programmed Cell Death 1 Ligand Protein 1; CD274 or B7-H1) is an immuno-coinhibitory member of the B7 family and functions as an immune checkpoint via its interaction with its receptors PD-1 (CD279) and CD80 (also known as B7-1). PD-L1 is expressed by hematopoietic cells such as T, B and dendritic cells and by parenchymal cells in response to cytokine (i.e. IFN-γ) induction. Ligation of PD-1 by PD-L1 (B7-H1; CD274) or PD-L2 (B7-DC; CD273) inhibits TCR-mediated T cell proliferation and production of IL-1, IL-4, IL-10 and IFN-γ and also inhibits BCR mediated signaling. PD-L1 binding to CD80 inhibits T cells proliferation. PD-1 deficient mice have a defect in peripheral tolerance and spontaneously develop autoimmune diseases. Soluble form of PD-L1 (sPD-L1) is expressed upon immune cells activation and can be secreted in the serum/plasma. Soluble checkpoints, such as sPD-1 or sPD-L1, are involved in positive or negative immune regulation and changes in their serum/plasma levels affect the development, prognosis and treatment of cancer. Increase of sPD-L1 or sPD-1 levels have been detected in patients with different types of cancer. This PD-L1 (human) ELISA kit is developed to detect specifically sPD-L1 (human) in different biological fluids.

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