CD274 [B7-H1/PD-L1] (human):Fc (human) (rec.)
Method: Human CD4+ T cells are activated with coated anti-CD3 (h) (2.5µg/ml) and incubated for 5 days with PDL1 [CD274] (human):Fc (human) (rec.) or Fc control protein (Prod. No. AG-35B-0007) (coated at 10µg/ml). As a positive control, VISTA (human):COMP (mouse) (rec.) (His) (Prod. No. AG-40B-0183) (coated at 10µg/ml) is used in the same conditions. IFNg is measured from cell supernatants using the Cymax IFN-g (human) ELISA Kit (Prod. No. YIF-LF-EK0288).
|Synonyms||PD-L1; Programmed Cell Death 1 Ligand Protein 1; PDCD1 Ligand 1; B7-H1; PDL1|
The extracellular domain of human CD274 [B7-H1] (aa 19-238) is fused to the N-terminus of the Fc region of human IgG1.
|Endotoxin Content||<0.06EU/μg protein (LAL test; Lonza).|
Reconstitute with 100 µl sterile water.
Add 1X PBS to the desired protein concentration.
|Formulation||Lyophilized from 0.2μm-filtered solution in PBS.|
|Protein Negative Control|
|Other Product Data||
NCBI reference NP_054862.1: CD274 (human)
|Declaration||Manufactured by Chimerigen.|
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||-20°C|
Avoid freeze/thaw cycles.
Centrifuge lyophilized vial before opening and reconstitution.
Stable for at least 1 year after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
|Product Specification Sheet|
Programmed death ligand 1 (PD-L1, B7-H1 or CD274) is a member of the growing B7 family of immune proteins that provide signals for both stimulating and inhibiting T cell activation. CD274 has been identified as one of two ligands for programmed death 1 (PD-1), a member of the CD28 family of immunoreceptors. CD274 is widely expressed in several organs such as heart, skeletal muscle, placenta and lung, and in lower amounts in thymus, spleen, kidney and liver. CD274 expression is upregulated in a small fraction of activated T and B cells and a much larger fraction of activated monocytes. CD274 expression is also induced in dendritic cells and keratinocytes after IFN-γ stimulation. CD274 expression is also upregulated in a variety of tumor cell lines. Interaction of CD274 with PD-1 results in inhibition of TCR mediated proliferation and cytokine production, suggesting an inhibitory role in regulating immune responses.. The CD274 - PD-1 pathway is involved in the negative regulation of some immune responses and may play an important role in the regulation of peripheral tolerance.