CD274 [B7-H1/PD-L1] (human):Fc (mouse) (rec.)

CHF 480.00
In stock
CHI-HF-211PDL1-C100100 µgCHF 480.00
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Product Details
Synonyms PD-L1; Programmed Cell Death 1 Ligand Protein 1; PDCD1 Ligand 1; B7-H1; PDL1
Product Type Protein
Properties
Source/Host HEK 293 cells
Sequence

The extracellular domain of human CD274 [B7-H1] (aa 19-238) is fused to the N-terminus of the Fc region of mouse IgG2a.

Crossreactivity Human
Biological Activity

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes.

MW ~70kDa (SDS-PAGE)
Purity ≥98% (SDS-PAGE)
Endotoxin Content <5EU/mg protein (LAL test; Lonza).
Reconstitution Reconstitute with 100 µl sterile water.
Add 1X PBS to the desired protein concentration.
Formulation Lyophilized from 0.2μm-filtered solution in PBS.
Protein Negative Control

Fc (mouse) IgG2a Control (rec.)

Other Product Data

NCBI reference NP_054862.1: CD274 (human)

Declaration Manufactured by Chimerigen.
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
MSDS Inquire
Product Specification Sheet
Datasheet Download PDF

Programmed death ligand 1 (PD-L1, B7-H1 or CD274) is a member of the growing B7 family of immune proteins that provide signals for both stimulating and inhibiting T cell activation. CD274 has been identified as one of two ligands for programmed death 1 (PD-1), a member of the CD28 family of immunoreceptors. CD274 is widely expressed in several organs such as heart, skeletal muscle, placenta and lung, and in lower amounts in thymus, spleen, kidney and liver. CD274 expression is upregulated in a small fraction of activated T and B cells and a much larger fraction of activated monocytes. CD274 expression is also induced in dendritic cells and keratinocytes after IFN-γ stimulation. CD274 expression is also upregulated in a variety of tumor cell lines. Interaction of CD274 with PD-1 results in inhibition of TCR mediated proliferation and cytokine production, suggesting an inhibitory role in regulating immune responses.. The CD274 - PD-1 pathway is involved in the negative regulation of some immune responses and may play an important role in the regulation of peripheral tolerance.

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