CD274 [B7-H1/PD-L1] (mouse):Fc (mouse) (rec.)
Method: T cells from C57BL/6 mice were activated with coated anti-CD3/CD28 and PD-L1-Fc or control antibody (10µg/ml). Plots illustrate the percentage of dividing cells by using anti-CD44 and the dye Cell Trace Violet (CTV). Picture Courtesy G. Guarda and A. Zenobi, Institute for Research in Biomedicine, Bellinzona (Switzerland).
|Synonyms||PD-L1; Programmed Cell Death 1 Ligand Protein 1; PDCD1 Ligand 1; B7-H1; PDL1|
The extracellular domain of mouse CD274 [B7-H1] (aa 19-237) is fused to the N-terminus of the Fc region of mouse IgG2a.
Inhibits antiCD3/CD28 antibody-induced proliferation of mouse T lymphocytes.
|Endotoxin Content||<0.06EU/μg protein (LAL test; Lonza).|
|Reconstitution||Reconstitute at 100μg/ml in sterile PBS.|
|Formulation||Lyophilized from 0.2μm-filtered solution in PBS.|
|Protein Negative Control|
|Other Product Data||
NCBI reference NP_068693.1: CD274 (mouse)
|Declaration||Manufactured by Chimerigen.|
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||-20°C|
Avoid freeze/thaw cycles.
Centrifuge lyophilized vial before opening and reconstitution.
Stable for at least 1 year after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
|Product Specification Sheet|
Programmed death ligand 1 (PD-L1, B7-H1 or CD274) is a member of the growing B7 family of immune proteins that provide signals for both stimulating and inhibiting T cell activation. CD274 has been identified as one of two ligands for programmed death 1 (PD-1), a member of the CD28 family of immunoreceptors. CD274 is widely expressed in several organs such as heart, skeletal muscle, placenta and lung, and in lower amounts in thymus, spleen, kidney and liver. CD274 expression is upregulated in a small fraction of activated T and B cells and a much larger fraction of activated monocytes. CD274 expression is also induced in dendritic cells and keratinocytes after IFN-γ stimulation. CD274 expression is also upregulated in a variety of tumor cell lines. Interaction of CD274 with PD-1 results in inhibition of TCR mediated proliferation and cytokine production, suggesting an inhibitory role in regulating immune responses.. The CD274 - PD-1 pathway is involved in the negative regulation of some immune responses and may play an important role in the regulation of peripheral tolerance.
- Programmed Death-1 Ligand 1 Interacts Specifically with the B7-1 Costimulatory Molecule to Inhibit T Cell Responses: M.J. Butte, et al.; Immunity 27, 111 (2007)
- Butyrophilin-like 2 regulates site-specific adaptations of intestinal γδ intraepithelial lymphocytes: C. Panea, et al.; Nature Commun. Biol. 4, 913 (2021)
- A newly intervention strategy in preeclampsia: Targeting PD-1/Tim-3 signaling pathways to modulate the polarization of decidual macrophages: X.H. Hu, et al.; FASEB J. 36, e22073 (2022)