CD40L (human) ELISA Kit

CHF 690.00
In stock
AG-45B-0018-KI0196 wellsCHF 690.00
NEW
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Product Details
Synonyms CD40 Ligand; CD154; Tumor Necrosis Factor Ligand Superfamily Member 5; TNFSF5; Gp39; TRAP
Product Type Kit
Properties
Application Set Quantitative ELISA
Specificity

Detects soluble human CD40L in serum, plasma and cell culture supernatant.

Crossreactivity Human
Quantity

1 x 96 wells

Sensitivity 20pg/ml
Range 0.03125 to 2ng/ml
Sample Type Cell Culture Supernatant
Plasma
Serum
Assay Type Sandwich
Detection Type Colorimetric
Other Product Data

UniProt link P29965: CD40L (human)

Declaration In collaboration with Suzhou Bright Scistar Biotechnology
Accession Number P29965
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage +4°C
Handling Advice After standard reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Plate and reagents should reach room temperature before use.
Use/Stability 12 months after the day of manufacturing. See expiry date on ELISA Kit box.
Documents
Manual Download PDF
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF

CD40 (also called, Tumor necrosis factor receptor superfamily member 5 or TNFRSF5), a transmembrane type I glycoprotein, belongs to the tumor necrosis factor (TNF) gene superfamily and behaves as a co-stimulatory molecule found on B cells, monocytes, antigen presenting cells, endothelial, smooth muscle cells, fibroblasts and platelets. Its ligand, CD40L (also called CD154), a type II transmembrane protein, is a 261 amino acid (aa) glycoprotein that forms homotrimers. CD40L is found on activated T cells, B cells, platelets, endothelial, epithelial and smooth muscle cells. Proteolytic cleavage can produce a soluble form of CD40L (sCD40L). Platelets are the main source of sCD40L. 

CD40-CD40L interaction is an essential signal for B cell proliferation, expression of activation markers, immunoglobulin production and isotype switching, formation of B memory cells and germinal centers. It also prevents apoptosis of germinal center B cells. Defective expression of CD40L in humans leads to an inability to produce isotypes other than IgM (hyper IgM syndrome) and to an absence of germinal centers. In dendritic cells, CD40 ligation induces more effective antigen, enhances T-cell stimulatory capacity and induces production of several inflammatory cytokines and chemokines. CD40-CD40L interactions are crucially involved in development of autoimmune disease and have also impact on growth regulation of certain carcinomas. Elevated levels of sCD40L have been observed in sera from patients with systemic lupus erythematosus (SLE), chronic lymphocytic leukemia (CLL) and unstable angina. 

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