AdipoGen Life Sciences

SARS-CoV-2 Neutralizing Antibodies Detection Kit

CHF 840.00
In stock
AG-48B-0002-KI0196 wellsCHF 840.00
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Product Details
Synonyms COVID-19 Neutralizing Antibodies Detection Kit; 2019-nCoV Neutralizing Antibodies Detection Kit; 2019-nCoV Spike Protein S1 (RBD) Neutralizing Antibodies Detection Kit
Product Type Kit
Application Set Qualitative ELISA

To measure the presence of neutralizing/blocking antibodies in human serum/plasma that inhibit the binding of the SARS-CoV-2 Spike (RBD) protein to its human receptor ACE2.

Crossreactivity Avian
Guinea pig

Contains sufficient materials for 96 reactions

Sample Type Plasma
Detection Type Colorimetric
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage +4°C
Handling Advice After standard reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Plate and reagents should reach room temperature before use.
Use/Stability 12 months after the day of manufacturing. See expiry date on ELISA Kit box.
Manual Download PDF
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF

Coronaviruses (CoVs) are enveloped non-segmented positive-sense single-stranded RNA viruses and can infect respiratory, gastrointestinal, hepatic and central nervous system of human and many other wild animals. Recently, a new severe acute respiratory syndrome β-coronavirus called SARS-CoV-2 has emerged, which causes an epidemic of acute respiratory syndrome (called coronavirus human disease 2019 or COVID-19). SARS-CoV-2 contains 4 structural proteins, including Envelope (E), Membrane (M), Nucleocapsid (N) and Spike (S), which is a transmembrane protein, composed of two subunits S1 and S2. The S1 subunit contains a receptor binding domain (RBD), which binds to the cell surface receptor Angiotensin-Converting Enzyme 2 (ACE2) present at the surface of epithelial cells, causing mainly infection of human respiratory cells.The SARS-CoV-2 Neutralizing Antibodies Detection Kit contains key reagents required to test the presence of functional neutralizing antibodies against SARS-CoV-2 present in the serum or plasma. It is an easy and fast alternative to the classical neutralization assay using Vero E6 cells. This Detection Kit is based on a colorimetric reaction, which measures the binding of the RBD of the Spike S protein from SARS-CoV-2 to its human receptor ACE2. The presence of neutralizing / blocking antibodies in the samples are detected by reduction of signal indicating the inhibition of the Spike-ACE2 binding. 

Product References
  1. Comparison of two commercial surrogate ELISAs to detect a neutralizing antibody response to SARS-CoV-2: K. Mueller, et al.; J. Virol. Methods 292, 114122 (2021)
  2. Unique autoantibody prevalence in long-term recovered SARS-CoV-2-infected individuals: H. Lingel, et al.; J. Autoimmun. 122, 102682 (2021)
  3. Evaluation of Two Rapid Lateral Flow Tests and Two Surrogate ELISAs for the Detection of SARS-CoV-2 Specific Neutralizing Antibodies: P. Girl, et al.; Front. Med. 9, 820151 (2022)
  4. Efficacy and impact of SARS‑CoV‑2 vaccination on cancer treatment for breast cancer patients: a multi‑center prospective observational study: M. Terada, et al.; Breast Cancer Res. Treat. 195, 311 (2022)
  5. Preexisting Humoral Immunity Cross-Reacting with SARS-CoV-2 Might Prevent Death Due to COVID-19 in Critical Patients: T. Yamashita, et al.; J. Clin. Med. 11, 3870 (2022)
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