AdipoGen Life Sciences

SARS-CoV-2 Inhibitor Screening Kit

CHF 780.00
In stock
AG-48B-0001-KI0196 wellsCHF 780.00
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Product Details
Synonyms COVID-19 Inhibitor Screening Kit; 2019-nCoV Inhibitor Screening Kit; 2019-nCoV Spike Protein S1 (RBD)/ACE2 Inhibitor Screening Kit
Product Type Kit
Application Set Compound Screening

To screen compounds that inhibit the binding of the viral protein SARS-CoV-2 Spike Protein (RBD) to its human receptor ACE2.

Crossreactivity Human

Contains sufficient materials for 96 reactions

Detection Type Colorimetric
Kit Contains

1 vial ACE2 (human) (rec.) (Biotin) (lyophilized) ----- 10 µg

1 vial SARS-CoV-2 Spike S (RBD):Fc (h) (rec.) (lyophilized) ----- 10 µg

1 vial HRP Labeled Streptavidin (lyophilized) ----- 2 µg

1 vial ACE2 (h), mAb (blocking) (AC384) ----- 20 µl

1 vial anti-human IgG (HRP) ----- 120 µl

1 MaxiSorp™flat-bottom 96 well plate ----- 6x16-well strips

2 bottles Wash Buffer 10x ----- 2 x 30 ml

1 bottle ELISA Buffer 10x ----- 30 ml

1 bottle Blocking Buffer ----- 24 ml

1 bottle TMB K-Blue Aqueous ----- 12 ml

1 bottle Stop Solution ----- 12 ml

2 silica gel minibags

2 plate sealers (plastic film)

Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage +4°C
Handling Advice After standard reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Plate and reagents should reach room temperature before use.
Use/Stability 12 months after the day of manufacturing. See expiry date on ELISA Kit box.
Manual Download PDF
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF

Coronaviruses (CoVs) are enveloped non-segmented positive-sense single-stranded RNA viruses and can infect respiratory, gastrointestinal, hepatic and central nervous system of human and many other wild animals. Recently, a new severe acute respiratory syndrome β-coronavirus called SARS-CoV-2 (or 2019-nCoV) has emerged, which causes an epidemic of acute respiratory syndrome (called coronavirus human disease 2019 or COVID-19).

SARS-CoV-2 contains 4 structural proteins, including Envelope (E), Membrane (M), Nucleocapsid (N) and Spike (S), which is a transmembrane protein, composed of two subunits S1 and S2. The S1 subunit contains a receptor binding domain (RBD), which binds to the cell surface receptor Angiotensin-Converting Enzyme 2 (ACE2) present at the surface of epithelial cells, causing mainly infection of human respiratory cells.

The SARS-CoV-2 Inhibitor Screening Kit contains key reagents required to facilitate identification of SARS-CoV-2 inhibitors. This inhibitor screen is based on a colorimetric ELISA kit, which measures the binding of the RBD of the Spike S protein from SARS-CoV-2 to its human receptor ACE2. This assay allows to identify and characterize the effect of different inhibitory molecules including antibodies or chemicals on the prevention of binding of SARS-CoV-2 virus to any ACE2-expressing cells.

Product References
  1. Gold Metallodrugs to Target Coronavirus Proteins: Inhibitory Effects on the Spike‐ACE2 Interaction and on PLpro Protease Activity by Auranofin and Gold Organometallics: M. Gil-Moles, et al.; Chem. Eur. J. 26, 15140 (2020)
  2. Pomegranate Peel Extract as an Inhibitor of SARS-CoV-2 Spike Binding to Human ACE2 Receptor (in vitro): A Promising Source of Novel Antiviral Drugs: A. Tito, et al.; Front. Chem. 9, 638187 (2021)
  3. Metallodrug Profiling against SARS‐CoV‐2 Target Proteins Identifies Highly Potent Inhibitors of the S/ACE2 interaction and the Papain‐like Protease PLpro: M. Gil-Moles, et al.; Chemistry 27, 17928 (2021)
  4. Pomegranate Pomace Extract with Antioxidant, Anticancer, Antimicrobial, and Antiviral Activity Enhances the Quality of Strawberry-Yogurt Smoothie: N.H. Alsubhi, et al.; Bioengineering 9, 735 (2022)
  5. In Vitro Screening and MD Simulations of Thiourea Derivatives against SARS-CoV‑2 in Association with Multidrug Resistance ABCB1 Transporter: M. Assad, et al.; ACS Omega 7, 47671 (2022)
  6. Montelukast and Telmisartan as Inhibitors of SARS-CoV-2 Omicron Variant: N. Mulgaonkar, et al.; Pharmaceutics 15, 1891 (2023)
  7. Aerosolized sulfated hyaluronan derivatives prolong the survival of K18 ACE2 mice infected with a lethal dose of SARS-CoV-2: M. Pavan, et al.; Eur. J. Pharmaceut. Sci. 187, 106489 (2023)
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