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Y-27632 . dihydrochloride

CHF 65.00
In stock
AG-CR1-3564-M0011 mgCHF 65.00
AG-CR1-3564-M0055 mgCHF 100.00
AG-CR1-3564-M01010 mgCHF 170.00
AG-CR1-3564-M02525 mgCHF 330.00
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Product Details
Synonyms (R)-(+)-trans-4-(1-Aminoethyl)-N-(4-pyridyl)cyclohexanecarboxamide dihydrochloride
Product Type Chemical

C14H21N3O . 2HCl

MW 247.3 . 72.9
CAS 129830-38-2 | 146986-50-7 (free base)
Purity Chemicals ≥98% (HPLC)
Contains approx. 1.4% water.
Appearance White to pale yellow solid.
Solubility Soluble in water (30mg /ml), DMSO (25mg/ml) or methanol (25mg/ml).
Identity Determined by 1H-NMR.
Smiles C[C@@H](N)C1CCC(CC1)C(=O)NC1=CC=NC=C1
Shipping and Handling
Shipping AMBIENT
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After reconstitution, prepare aliquots and store at -20°C.
Keep under inert gas.
Protect from light.
Use/Stability Stable for at least 2 years after receipt when stored at -20°C.
Stock solutions are stable for at least 3 months when stored at -20°C.
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Product Specification Sheet
Datasheet Download PDF
  • Potent, cell permeable, selective and ATP-competitive Rho-associated protein kinases inhibitor, including p160ROCK, ROCK-II and PRK2 inhibitor [1,3-5].
  • Tumor cell invasion and metastasis suppressor [2, 15].
  • Smooth muscle relaxant [1, 6].
  • Decreases liver fibrosis by hepatic stellate cell growth inhibition [7, 8].
  • Antinociceptive [9].
  • Blocks generation of inflammatory cytokines [10].
  • Stem cell research modulator [11-14, 16].
  • Prevents apoptosis and enhances the survival and cloning efficiency of dissociated human embryonic stem (hES) cells without affecting their pluripotency. Increases survival rate of hES cells undergoing cryopreservation [11-14, 16].
Product References
  1. Calcium sensitization of smooth muscle mediated by a Rho-associated protein kinase in hypertension: M. Uehata, et al.; Nature 389, 990 (1997)
  2. An essential part for Rho-associated kinase in the transcellular invasion of tumor cells: K. Itoh, et al.; Nat. Med. 5, 221 (1999)
  3. Use and properties of ROCK-specific inhibitor Y-27632: S. Narumiya, et al.; Meth. Enzymol. 325, 273 (2000)
  4. Specificity and mechanism of action of some commonly used protein kinase inhibitors: S.P. Davies, et al.; Biochem. J. 351, 95 (2000)
  5. Pharmacological properties of Y-27632, a specific inhibitor of rho-associated kinases: T. Ishizaki, et al.; Mol. Pharmacol. 57, 976 (2000)
  6. Y-27632 potentiates relaxant effects of beta 2-adrenoceptor agonists in bovine tracheal smooth muscle: T. Nakahara, et al.; Eur. J. Pharmacol. 389, 103 (2000)
  7. A p160ROCK-specific inhibitor, Y-27632, attenuates rat hepatic stellate cell growth: H. Iwamoto, et al.; J. Hepatol. 32, 762 (2000)
  8. Inhibition of intrahepatic metastasis of human hepatocellular carcinoma by Rho-associated protein kinase inhibitor Y-27632: M. Takamura, et al.; Hepatology 33, 577 (2001)
  9. Rho-kinase inhibitor, Y-27632, has an antinociceptive effect in mice: K. Buyukafsar, et al.; Eur. J. Pharmacol. 541, 49 (2006)
  10. Novel Rho kinase inhibitors with anti-inflammatory and vasodilatory activities: C. Doe, et al.; JPET 320, 89 (2007)
  11. A ROCK inhibitor permits survival of dissociated human embryonic stem cells: K. Watanabe, et al.; Nat. Biotechnol. 25, 681 (2007)
  12. The ROCK inhibitor Y-27632 enhances the survival rate of human embryonic stem cells following cryopreservation: X. Li, et al.; Stem Cells Dev. 17, 1079 (2008)
  13. Effect of Rho-associated kinase (ROCK) inhibitor Y-27632 on the post-thaw viability of cryopreserved human bone marrow-derived mesenchymal stem cells: B.C. Heng; Tissue Cell 41, 376 (2009)
  14. Effect of ROCK inhibitor Y-27632 on normal and variant human embryonic stem cells (hESCs) in vitro: its benefits in hESC expansion: K. Gauthaman, et al.; Stem Cell Rev. 6, 86 (2010)
  15. Pharmacological inhibition of Rho-kinase signaling with Y-27632 blocks melanoma tumor growth: A. Routhier, et al.; Oncol. Rep. 23, 861 (2010)
  16. The ROCK inhibitor Y-27632 improves recovery of human embryonic stem cells after fluorescence-activated cell sorting with multiple cell surface markers: N. Emre, et al.; PLoS One 5, e12148 (2010)
  17. Full paper viscoelastic hydrogels for interrogating pancreatic cancer-stromal cell interactions: F-Y. Lin, et al.; Materials Today Bio in press (2023)
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