anti-CD8-alpha (mouse), mAb (53-6) (preservative free)

Figure 1: Binding of anti-mCD8alpha (53-6.72) +GAR/FITC to balb-c splenocytes. Method: Tested for binding to ACK lysed murine splenocytes in FACS. Five x 10^5 splenocytes per tube were washed and pre-incubated with 20ul of 300ug/ml murine IgG (to reduce non-specific binding) after which they were incubated 45 minutes on ice with 80ul of anti-CD8 antibody diluted to 10ug/ml. Cells were washed twice and incubated with 2nd reagent Goat anti-Rat IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. An 8.8 % sub population of the cells stained positive with a mean shift of 1.6 log10 fluorescent units when compared to background.