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Ancell
anti-CD40 (human), mAb (EA-5) (APC)
585
CHF
CHF 585.00
In stock
ANC-300-060120 testsCHF 585.00
Figure 1: Binding of anti-CD40/APC to human Raji cells. Methods: Five x 10^5 human Raji cells per tube were incubated 45 min on ice with 80µl of anti-CD40/APC at a dilution factor of 1:50 (10µg/ml). Cells were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 2.94 log10 fluorescent units when compared to a Mouse IgG1/R-PE negative control (#ANC-278-050). Binding was blocked when cells were pre-incubated 10 min with 20µl of 0.5mg/ml anti-CD40 antibody (#ANC-300-020).
| Product Details | |
|---|---|
| Synonyms | CD40L Receptor; CDw40; Tumor Necrosis Factor Receptor Superfamily Member 5; TNFRSF5 |
| Product Type | Monoclonal Antibody |
| Properties | |
| Clone | EA-5 |
| Isotype | Mouse IgG1 |
| Immunogen/Antigen | Murine L cells transfectants expressing human CD40. |
| Label/Conjugates | APC |
| Application |
ELISA |
| Crossreactivity | Human |
| Specificity |
Antibody clone EA-5 recognizes the human CD40 molecule. |
| Purity | Protein A purified. |
| Formulation | 50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative). |
| Isotype Negative Control | |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| Declaration | Manufactured by Ancell Corporation. |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | +4°C |
| Handling Advice |
Do not freeze. Protect from light. |
| Use/Stability | Stable for at least 1 year after receipt when stored at +4°C. |
| Documents | |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
CD40 belongs to the TNF-receptor superfamily and is essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development and germinal center formation. The interaction of CD40-CD40L is necessary for amyloid-β-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. CD40 is constitutively expressed by antigen presenting cells, including dendritic cells, B cells and macrophages. Consistent with its widespread expression on normal cells, CD40 is also expressed on a wide range of tumor cells, including non-Hodgkin's and Hodgkin's lymphomas, myeloma and some carcinomas including nasopharynx, bladder, cervix, kidney and ovary.
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