anti-CD152 [CTLA-4] (human), mAb (ANC152.2/8H5) (APC)

Figure 1: Binding of anti-CD152/APC to recombinant CD152-coated CML beads. Methods: Anti-CD152/APC was tested for binding to CD152-muIg-coated 4µ CML beads in FACS (Inquire). Five x 10^5 CD152-muIg coated beads per tube were spun down and incubated 45 min at 4°C with 80µl of anti-CD152/APC at a 1:50 dilution (10µg/ml). Tubes were washed twice, fixed and analyzed by FACS. Beads stained positive with a mean shift of 1.92 log10 fluorescent units when compared to a Mouse IgG1/APC Isotype control (#ANC-278-060). Binding was blocked when beads were pre-incubated 10 min with 20µl of 0.5mg/ml anti-CD152 antibody (#ANC-359-020).