Ancell

CD244 [2B4] (human)-muIg Fusion Protein (Biotin)

CHF 669.00

In stock

ANC-544-03025 µgCHF 669.00

Figure 1: CD244-muIg/Biotin was tested for binding to cell surface CD48 on Raji cells. 5x10^5 cultured Raji cells per tube were washed and incubated 45 min on ice with 80µl of CD244-muIg/Biotin at a concentration of 10µg/ml. Cells were washed twice and incubated with 2nd reagent Streptavidin/R-PE (ANC-253-050), after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.79 log10 fluorescent units when compared to a muIgFc/Biotin negative control (ANC-581-030). Binding was blocked when cells were pre-blocked 10 min with 20µl of anti-CD48 antibody (Clone 5-4.8, ANC-199-020) at a concentration of 0.5mg/ml.

Specifications / Handling

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Product Details
Synonyms	Natural Killer Cell Receptor 2B4; NK Cell Activation-inducing Ligand; NAIL; NKR2B4; SLAMF4
Product Type	Protein
Properties
Source/Host	CHO cells
Sequence	A soluble molecule consisting of murine CD8 alpha signal peptide residual amino acids and linker the mature extracellular domain of human CD244 (199aa) and murine IgG2a Fc+hinge regions.
Crossreactivity	Human
Application	ELISA Flow Cytometry
Label/Conjugates	Biotin
Specificity	Binds to recombinant CD48 in EIA and cell surface CD48 on Raji cell surface in FACS.
MW	~135kDa (native dimer) ~70kDa (SDS-PAGE, reduced)
Purity Detail	Affinity & SEC purified.
Concentration	0.5mg/ml
Accession Number	Q9BZW8
Formulation	50mM Sodium Phosphate pH 7.5, 100mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% sodium azide.
Protein Negative Control	MuIg Control Protein (Biotin)
Other Product Data	Click here for Original Manufacturer Product Datasheet Our product description may differ slightly from the original manufacturers product datasheet.
Declaration	Manufactured by Ancell Corporation.
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	+4°C
Handling Advice	Avoid freeze/thaw cycles.
Use/Stability	Stable for at least 6 months after receipt when stored at +4°C.
Documents
MSDS	Inquire
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

CD244 (2B4; SLAMF4) is a 66kDa member of the CD2/SLAM related receptor family (SRR). It is expressed on NK, T cell subsets, monocytes and eosinophils. It is expressed at high levels on leuikemia initiating cells, and plays a role in their proliferative potential. In mice there are two transcription variants, a long form and a short form of CD244 each of which have distinct signaling properties. In humans, only the long form is expressed. Decreased CD244 expression on monocytes of SLE patients correlated (negatively) with autoantibody titer. CD244 mediated function in immune response can vary greatly dependant on the context of its ligation.

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