Ancell

anti-CD244 (human), mAb (ANC2B4/3B12) (FITC)

CHF 423.00

In stock

ANC-259-040120 testsCHF 423.00

Figure 1: 5x10^5 ficoll prepared human PBMC were washed and pre-incubated 10 min with 20µl of 250µg/ml human IgG (to block non-specific binding), after which they were incubated 45 min on ice with 80µl of anti-CD244/FITC at a 1:50 dilution factor (10µg/ml). Cells were washed three times, fixed and analyzed by FACS. A 46% sub population of the cells stained positive with a mean shift of 1.52 log10 fluorescent units when compared to a Mouse IgG1/FITC negative control (ANC-278-040). Binding was blocked when cells were pre-incubated 10 min with 20µl of 0.5mg/ml anti-CD3 antibody (ANC-144-020).

Specifications / Handling

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Product Details
Synonyms	Natural Killer Cell Receptor 2B4; NK Cell Activation-inducing Ligand; NAIL; NKR2B4; SLAMF4
Product Type	Monoclonal Antibody
Properties
Clone	ANC2B4/3B12
Isotype	Mouse IgG1κ
Source/Host	Purified from concentrated hybridoma tissue culture supernatant.
Immunogen/Antigen	Recombinant soluble human CD244.
Label/Conjugates	FITC
Application	ELISA Flow Cytometry
Crossreactivity	Human
Specificity	Binds to a CD244 epitope that is distinct from the binding sites of clone ANC244.8 (ANC-239-020) and clone C1.7.
Purity Detail	Protein A purified.
Formulation	50mM Sodium Phosphate pH 7.5, 100mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
Isotype Negative Control	Mouse IgG1 Isotype Control (FITC)
Other Product Data	Click here for Original Manufacturer Product Datasheet Our product description may differ slightly from the original manufacturers product datasheet.
Accession Number	Q9BZW8
Declaration	Manufactured by Ancell Corporation.
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	+4°C
Handling Advice	Avoid freeze/thaw cycles. Protect from light.
Use/Stability	Stable for at least 1 year after receipt when stored at +4°C.
Documents
MSDS	Inquire
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

CD244 (2B4; SLAMF4) is a 66kDa member of the CD2/SLAM related receptor family (SRR). It is expressed on NK, T cell subsets, monocytes and eosinophils. It is expressed at high levels on leuikemia initiating cells, and plays a role in their proliferative potential. In mice there are two transcription variants, a long form and a short form of CD244 each of which have distinct signaling properties. In humans, only the long form is expressed. Decreased CD244 expression on monocytes of SLE patients correlated (negatively) with autoantibody titer. CD244 mediated function in immune response can vary greatly dependant on the context of its ligation.

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