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BellBrook
Transcreener OAS FP Assay
CHF 0.00
In stock
BBL-3027-1K1000 assaysCHF 1'234.00
BBL-3027-10K10000 assaysCHF 6'497.00
BBL-3027-100K10 x 10000 assaysINQ
Figure 1: Schematic Overview: Detection of 2-5A to Measure OAS Enzymatic Activity.
| Product Details | |
|---|---|
| Synonyms | 2'-5'-Oligoadenylate Synthase |
| Product Type | Kit |
| Properties | |
| Application Set | Compound Screening |
| Crossreactivity | All |
| Quantity |
1000 assays/384wells (BBL-3027-1K) 10000 assays/384wells (BBL-3027-10K) Note: The exact number of assays depends on enzyme reaction conditions. |
| Sensitivity | Excellent data quality (Z’ ≥ 0.7) and signal (≥100 mP polarization shift) for determining OAS activity by measuring 2-5A production. |
| Detection Type | Fluorescent polarization |
| Kit Contains |
AMP2/GMP2 Antibody, AMP2/GMP2 Alexa Fluor® 633 Tracer, 2-5A–AMP Coupling Enzyme, ATP, AMP, dsRNA, OAS Buffer 10X Note: The plates are not included, please see manual for material not provided but necessary for assay. |
| Other Product Data |
Click here for Complete Information from the Original Manufacturer
Measure Enzymatic Activity of OAS1, OAS2, or OAS3. Designed as in homogenous, one-step format to enable reproducibility. Robust Assay Z’ > 0.7 under initial velocity conditions. Far-red fluorescent readouts minimize compound interference. A safe, non-radioactive method. |
| Declaration | Manufactured by BellBrook Labs. |
| Shipping and Handling | |
| Shipping | DRY ICE |
| Short Term Storage | -20°C |
| Long Term Storage | -20°C |
| Handling Advice | Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| Manual |
 Download PDF |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
The Transcreener® high throughput screening (HTS) assay platform overcomes the need for time-consuming, one-off assay development for individual members within a group transfer enzyme family by utilizing a single set of assay reagents that detect an invariant product. The generic nature of the Transcreener® HTS Assay platform eliminates delays involved in assay development for new HTS targets and greatly simplifies compound and inhibitor profiling across multiple target families. Transcreener assays have been validated with major HTS instruments to determine the optimal filters and settings for maximal assay performance. Application notes with all important details are available. The Transcreener OAS Assay measures the 2-5A produced by OAS1 (also known as 2'-5'-oligoadenylate synthetase 1) OAS2, or OAS3. By measuring the production of OAS researchers can effectively determine the activity of OAS enzymes. The assay provides a powerful tool to screen compound libraries for OAS modulators to help find new therapeutics for a variety of different conditions including autoimmune diseases.
The Role of OAS1 In Innate Immunity: Double-stranded RNA enters the cell from viral infection. As the first line of defense in the innate immune system, OAS1 is activated by viral double-stranded RNA. Upon activation, OAS1 synthesizes 2-5A from ATP which in turn activates RNase L. The result of this signaling event cleaves both viral and host RNA and in turn inhibition of protein synthesis in the cell, thus ending the replication of invading RNA viruses. Mutation or inadvertent stimulation can produce autoimmune conditions by activating the pathway inconsistently even while not in the presence of viral invaders.
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