Enzolution™ DDX3 ATPase TR-FRET Assay System

The Enzolution DDX3 ATPase Assay System provides researchers with an assay tool that will simplify and advance their exploration of novel DDX3/innate immunity modulators. DDX3, a DEAD Box helicase protein characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), is an ATP-dependent RNA helicase that unwinds RNA-RNA and RNA-DNA duplexes. The assay system includes DDX3 enzyme, RNA substrate, assay plates, and buffers to generate a DDX3 enzymatic reaction to produce ADP. The Transcreener ADP TR-FRET Assay (sold separately) is a far-red, competitive assay that directly measures ADP production to determine enzymatic activity with a TR-FRET readout. The Transcreener ADP TR-FRET Assay is not included in the Enzolution Assay System but is available for separate purchases. The Transcreener ADP assay is designed for HTS, Hit-to-Lead, SAR, and general discovery.

When purchasing the DDX3 TR-FRET Assay System, it is important to choose the assay system readout corresponding to the TR-FRET configuration of your Transcreener ADP² Assay Kit!