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BellBrook
Enzolution SIRT3 TR-FRET Assay System
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BBL-3052-TR-1K1000 assaysCHF 762.00
BBL-3052-TR-10K10000 assaysCHF 3’076.00
BBL-3052-TR-100K10 x 10000 assaysINQ
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Figure 1: Schematic overview of the Enzolution™ SIRT3 TR-FRET Assay System with the Transcreener OAADPr SIRT Assay.
| Product Details | |
|---|---|
| Synonyms | SIRT3; NAD-dependent Deacetylase Sirtuin-3; SIR2-like Protein 3 |
| Product Type | Kit |
| Properties | |
| Application Set | Compound Screening |
| Crossreactivity | Human |
| Quantity |
1'000 assays/384wells (BBL-3052-TR-FRET-1K) 10'000 assays/384wells (BBL-3052-TR-FRET-10K) 100'000 assays/384wells (BBL-3052-TR-FRET-100K) Note: The exact number of assays depends on enzyme reaction conditions. |
| Detection Type | TR-FRET |
| Kit Contains |
- SIRT3 (human) (rec.) Enyzme - H3K9Ac Peptide, 10mM - Enzyme Assay Buffer G, 10X - MnCl2, 1M - 384-Well Low Volume Assay Plates (Corning#4513; sold under #BBL-2231 or BBL-2232) |
| Other Product Data |
Click here for Complete Information from the Original Manufacturer Our product description may differ slightly from the original manufacturer's product datasheet. |
| Declaration | Manufactured by BellBrook Labs. |
| Accession Number | Q9NTG7 |
| Shipping and Handling | |
| Shipping | DRY ICE |
| Short Term Storage | -20°C |
| Long Term Storage | -20°C |
| Handling Advice | Avoid freeze/thaw cycles. |
| Documents | |
| Manual |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
The Enzolution™ SIRT3 Assay System is intended for use with the Transcreener® OAADPr SIRT Assay Kits (Parts #3046 and #3047) to measure the enzymatic activity of SIRT3 (NAD-dependent protein deacetylase sirtuin 3). SIRT3 catalyzes transfer of acetyl groups from lysine residues to NAD, yielding the unacetylated peptide, O-acetyl-ADP ribose (OAADPr), and nicotinamide as products (Figure 1). The assay relies on a Coupling Enzyme (CE) to convert OAADPr into AMP, which is then detected using a far-red, competitive fluorescence polarization (FP) or time-resolved Förster-resonance-energytransfer (TR-FRET) assay. The assay has been optimized and extensively validated for high throughput screening (HTS) and inhibitor dose response measurements using most multimode plate readers.
The Enzolution SIRT3 Assay System provides all reagents required to screen and profile SIRT3 inhibitors when used with the Transcreener OAADPr SIRT Assay Kits, including purified, human SIRT3 (aa 101-399) and acetylated peptide, H3K9Ac (1-21). The protocol is configured for 384-well plates; use of different multi-well plate formats will require adjustment of reagent concentrations utilized in the assay.
When purchasing the Enzolution Assay System, it is important to choose the assay system readout that corresponds to the TR-FRET configuration of your Transcreener OAADPr SIRT Assay.
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