N-(5-Fluoresceinyl)-maleinimide

CHF 211.00
In stock
CDX-F0004-M05050 mgCHF 211.00
CDX-F0004-M500500 mgCHF 1'730.00
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Product Details
Synonyms N-(5-Fluoresceinyl)-maleimide; Fluorescein-​5-​maleimide; 1-(3',6'-Dihydroxy-3-oxo-3H-spiro[2-benzofuran-1,9'-xanthen]-5-yl)-1H-pyrrole-2,5-dione
Product Type Chemical
Properties
Formula C24H13NO7
MW 427.36
CAS 75350-46-8
Source/Host Chemicals Synthetic.
Purity Chemicals ≥90% (HPLC)
Appearance Orange to brown solid.
Solubility Soluble in DMF.
Identity Determined by 1H-NMR.
Declaration Manufactured by Chemodex.
Other Product Data

Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.

InChi Key AYDAHOIUHVUJHQ-UHFFFAOYSA-N
Smiles OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=CC(=C3)N1C(=O)C=CC1=O)C1=C(O2)C=C(O)C=C1
Shipping and Handling
Shipping AMBIENT
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice Protect from light and moisture.
Use/Stability Stable for at least 2 years after receipt when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF

Green fluorescent thiol-reactive dye (Ex/Em: 494/519nm) for bioconjugation, widely used as protein and peptide fluorescent labeling reagent, easily conjugating fluorescein. The reaction requires very mild condition and is highly specific. The maleimide group reacts optimally with sulfhydryl groups on cysteine side chains at pH 7, forming a stable thioether bond. The compound can be used to label proteins for the detection of conformational changes, assembly of multisubunit complexes, and ligand-binding processes.

Product References

(1) Anal. Lett. 18, 393 (1985) | (2) Biochem.; 30, 2113 (1991)

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