Cookie Policy: This site uses cookies to improve your experience. You can find out more about our use of cookies in our Privacy Policy. By continuing to browse this site you agree to our use of cookies.
Chemodex
Fluorescein (free acid)
Product Details | |
---|---|
Synonyms | Acid Yellow 73; NSC 667256; 3',6'-Fluorandiol |
Product Type | Chemical |
Properties | |
Formula | C20H12O5 |
MW | 332.31 |
CAS | 2321-07-5 |
Source/Host Chemicals | Synthetic |
Purity Chemicals | ≥99% (HPLC) |
Appearance | Red powder. |
Solubility | Soluble in acetone or DMSO. Insoluble in water or chloroform. |
Identity | Determined by NMR. |
Declaration | Manufactured by Chemodex. |
Other Product Data |
Click here for Original Manufacturer Product Datasheet |
InChi Key | GNBHRKFJIUUOQI-UHFFFAOYSA-N |
Smiles | OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=CC=C3)C1=CC=C(O)C=C1O2 |
Shipping and Handling | |
Shipping | AMBIENT |
Short Term Storage | +4°C |
Long Term Storage | +4°C |
Handling Advice |
Keep cool and dry. Protect from light and moisture. |
Use/Stability | Stable for at least 2 years after receipt when stored at +4°C. |
Documents | |
MSDS | Inquire |
Product Specification Sheet | |
Datasheet | Download PDF |
Fluorescein is a fluorophore commonly used in microscopy, flow cytometry forensics, serology to detect latent blood stains, and in dye tracing (attached to certain biologically active molecules). This fluorescent dye/probe has spectral data of λex 490 nm| λem 514 nm in 0.1 M Tris pH 8.0 and is strongly pH dependent. It has an isosbestic point (equal absorption for all pH values) at 460 nm. Fluorescein derivatives are used in different applications in cell biology.
(1) R.F. Chen; Arch. Biochem. Biophys. 133, 263 (1969) | (2) R. Sjöback, et al.; Spectrochim. Acta. A. Mol. Biomol. Spectrosc. 51, L7 (1995) | (3) B.L. Drees, et al.; J. Biol. Chem. 271, 32168 (1996) | (4) M. Taki, et al.; Prot. Eng. Des. Select. 17, 119 (2004)