Fluorescein-5-thiosemicarbazide

CHF 118.00
In stock
CDX-F0005-M05050 mgCHF 118.00
CDX-F0005-M100100 mgCHF 210.00
CDX-F0005-M500500 mgCHF 863.00
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Product Details
Synonyms 5-FTSC; FTSC, FTZ; N-(3',6'-Dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl)-hydrazinecarbothioamide
Product Type Chemical
Properties
Formula C21H15N3O5S
MW 421.43
CAS 76863-28-0
Source/Host Chemicals Synthetic.
Purity Chemicals ≥97% (NMR)
Appearance Orange to brown powder.
Solubility Soluble in water or DMF.
Identity Determined by 1H-NMR.
Declaration Manufactured by Chemodex.
Other Product Data Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.
InChi Key OIMMDIXLTJAVTF-UHFFFAOYSA-N
Shipping and Handling
Shipping AMBIENT
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice Protect from light and moisture.
Use/Stability Stable for at least 2 years after receipt when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF

Hydrazide derivative of fluorescein. An amine-containing cell impermeant fluorescent probe that can be reversibly coupled to aldehydes and ketones on diverse molecules. This forms a Schiff base - which can be further reduced to generate stable amine derivatives by treatment with reducing agents such as sodium borohydride (NaBH4) or sodium cyanoborohydride (NaCNH3). Carboxylic acids of proteins and other water-soluble biopolymers can also be coupled to this fluorescein derivative in aqueous solution using water-soluble carbodiimides such as EDAC. Fluorescent tag for labeling of cell-surface functional groups (glycophorins) and many other diverse molecules, including DNA, RNA, polysaccharides, sialylated glycoproteins, carbonylated proteins, carbonyl derivatives, and N-acetylneuraminic acid. Useful for determining protein and peptide topology on the cell surface. This probe has been used for directly imaging of mucin-type O-linked glycoproteins within living cells, for imaging polysaccharides transported in living cells and for fluorescence labeling of short RNA by oxidation at the 3′-End. Spectral data: λex=492nm; λem=516nm (in 0.1 M Tris pH9.0).

Product References

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