Merocyanin 540

CHF 89.00
In stock
CDX-M0033-M100100 mgCHF 89.00
CDX-M0033-M500500 mgCHF 357.00
CDX-M0033-G0055 gCHF 2'144.00
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Product Details
Synonyms MC-540
Product Type Chemical
Properties
Formula

C26H32N3NaO6S2

MW 569.67
CAS 62796-23-0
RTECS DM4866500
Source/Host Chemicals Synthetic.
Purity Chemicals ≥90% (HPLC)
Appearance Deep violet powder.
Solubility Soluble in DMSO, ethanol, methanol or water.
Identity Determined by 1H-NMR.
Declaration Manufactured by Chemodex.
Other Product Data

Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.

InChi Key OSQUFVVXNRMSHL-LTHRDKTGSA-M
Smiles [Na+].CCCCN1C(=O)C(=C\C=C\C=C2/OC3=CC=CC=C3N2CCCS([O-])(=O)=O)C(=O)N(CCCC)C1=S
Shipping and Handling
Shipping AMBIENT
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice Protect from light and moisture.
Use/Stability Stable for at least 2 years after receipt when stored at -20°C.
Documents
MSDS Inquire
Product Specification Sheet
Datasheet Download PDF

Merocyanine 540 (MC-540) is an anionic, lipophilic sensitive fluorescent probe for membrane potential. It is a sodium salt of sulfonic acid, with a chain of four conjugated methine groups, a substituted benzoxazole ring at one end, and substituted thiobarbituric acid at the other end. MC-540 was originally developed as a photosensitizer for photographic emulsions. However, its main importance today is in biology and medicine. It is widely used as a biological probe in membrane studies (fluorescent probe for studies of membrane systems, optical probe for transmembrane potentials), for characterization of the multidrug resistance phenotype, for photo-induced cytotoxicity as a highly effective drug against human malignant cells (photodynamic purging of neoplastic cells in bone marrow explants - leukemia, treatment of lymphomas and metastatic neuroblastomas) and other diseases (malaria, Herpes simplex, HIV). MC-540 has been shown to be a selective stain for leukemic and immature hemopoietic cells in flow cytometry. Spectral Data: Ex/Em: 560/579nm (see Reference 10 for excitation of different solvents). MC-540 is spectrally similar to Alexa Fluor 546, Cy3 (Cyanine-3), Sulforhodamine B, TAMRA and Sulforhodamine B sulfonyl chloride. 

Product References

(1) K.W. Kinnally, et al.; Biochem. 17, 3419 (1978) | (2) T.G. Easton, et al.; Cell 13, 475 (1978) | (3) P.I. Lelkes & I.R. Miller; J. Membr. Biol. 52, 1 (1980) | (4) K. Masamoto, et al.; Biochim. Biophys. Acta 638, 108 (1981) | (5) P. Williamson, et al.; Biochim. Biophys. Acta 732, 387 (1983) | (6) N.S. Dixit & R.A. Mackay; JACS 105, 2928 (1983) | (7) F. Sieber, et al.; Photochem. Photobiol. 46, 1035 (1987) | (8) F. Belloc, et al.; Cytometry 9, 19 (1988) | (9) M. Castedo, et al.; J. Immunol. 157, 512 (1996) | (10) C. Pakanyi, et al.; In: Analytical Use of Fluorescent Probes in Oncology p371-382 (1996) | (11) H.A. Wilson-Ashworth, et al.; Bioph. J. 91, 4091 (2006) | (12) S.K. Krumova, et al.; Biochim. Biophys. Acta, Biomemb. 1778, 2823 (2008) | (13) D. Mukherjee, et al.; Colloids Surf B Biointerfaces 196, 111363 (2020)

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