|Purity Chemicals||≥98% (HPLC)|
|Appearance||Deep blue, violet crystals.|
|Solubility||Soluble in DMSO. Poor solubility in water (1µM).|
|Identity||Determined by NMR.|
|Declaration||Manufactured by Chemodex.|
|Other Product Data||
Click here for Original Manufacturer Product Datasheet
Solubility: Due to the low solubility in water, we recommend preparing a JC-10 stock solution in DMSO (1mg/ml) which then can be further diluted in the aqueous buffer of choice. The DMSO stock solution of the compound is stable for at least 1 month when stored at -20°C. Avoid repeated freeze-thaw cycles which can damage the compound. Prepare the working solution on the day of use. The working solution can not be restored or frozen and should be used within one day.
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||-20°C|
Keep cool and dry.
Protect from light and moisture.
|Use/Stability||Stable for at least 2 years after receipt when stored at -20°C.|
|Product Specification Sheet|
Derivative of JC-1 useful for determining mitochondrial membrane potential by flow cytometry, fluorescence microscopy and in microplate-based fluorescent assays. Accumulates in mitochondria, selectively generating an orange J-aggregate emission profile (570 nm) in healthy cells. Upon cell injury, as membrane potential decreases, JC-10 monomers are generated, resulting in a shift to green emission (520 nm). Shows improved solubility compared to JC-1 in aqueous media and an ability to detect subtler changes in mitochondrial membrane potential loss. Spectral data: λex 510 nm| λem 525.
(1) L. Donaghy, et al.; PLoS One 7, e46594 (2012) | (2) C.C. Chen, et al.; Drug Des. Dev. Ther. 8, 459 (2014) | (3) S. Yokokura, et al.; BMC Cancer 26, 882 (2014) | (4) C. Filippi, et al.; Nanotoxicology 9, 126 (2015)