JC-10 (high purity)
|Purity Chemicals||≥98% (HPLC)|
|Appearance||Deep blue to violet solid.|
|Solubility||Soluble in water or DMSO.|
|Identity||Determined by 1H-NMR.|
|Other Product Data||Application Protocols for JC-1: See literatures 1-3.|
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||-20°C|
Keep cool and dry.
Protect from light.
|Use/Stability||Stable for at least 2 years after receipt when stored at -20°C.|
|Product Specification Sheet|
JC-10 is superior analog of JC-1, useful for determining mitochondrial membrane potential (MMP) in cells by flow cytometry, fluorescence microscopy and in microplate-based fluorescent assays. JC-10 is a cationic, lipophilic dye that accumulates in mitochondria of cells with a polarized mitochondrial membrane, selectively generating an orange J-aggregate emission profile (λex = 540 nm/λem = 590 nm) in healthy cells. Upon cell injury or cell death, as membrane potential decreases, which results in the failure to retain JC-10 aggregates in the mitochondria and JC-10 monomers are generated resulting in a shift to green emission (λex = 490 nm/λem = 525 nm). JC-10 shows improved solubility compared to JC-1 in aqueous media and an ability to detect subtler changes in mitochondrial membrane potential loss. JC-10 allows for qualitative visualization (shift from orange to green fluorescence) and quantitative detection (fluorescence intensity ratio) of mitochondrial membrane potential changes. Wavelength Maxima: λex = 510 nm | λem = 525 nm.
- Flow cytometric analysis of mitochondrial membrane potential using JC-1: A. Cossarizza & S. Salvioli; Curr. Protoc. Cytom. Chapter 9, Unit 9.14 (2001) (JC-1 Protocol)
- Polychromatic analysis of mitochondrial membrane potential using JC-1: E. Lugli, et al.; Curr. Protoc. Cytom. Chapter 7, Unit 7.32 (2007) (JC-1 Protocol)
- Labeling mitochondria with JC-1: B. Chazotte; Cold Spring Harb. Protoc. 2011, (2011) (JC-1 Protocol)
- Reactive oxygen species in unstimulated hemocytes of the pacific oyster Crassostrea gigas: A mitochondrial involvement: L. Donaghy, et al.; PLoS One 7, e46594 (2012)
- Improving anticancer efficacy of (-)-epigallocatechin-3-gallate gold nanoparticles in murine B16F10 melanoma cells: C.C. Chen, et al.; Drug Des. Dev. Ther. 8, 459 (2014)
- Calmodulin antagonists induce cell cycle arrest and apoptosis in vitro and inhibit tumor growth in vivo in human multiple myeloma: S. Yokokura, et al.; BMC Cancer 26, 882 (2014)
- Toxicology of ZnO and TiO2 nanoparticles on hepatocytes: impact on metabolism and bioenergetics: C. Filippi, et al.; Nanotoxicology 9, 126 (2015)
- Neurotoxicity of a pyrethroid pesticide deltamethrin is associated with the imbalance in proteolytic systems caused by mitophagy activation and proteasome inhibition: T. Hirano, et al.; Toxicol. Appl. Pharmacol. in press (2021)
- Chemically Defined Xeno- and Serum-Free Cell Culture Medium to Grow Human Adipose Stem Cells: S. Panella, et al.; Cells 10, 466 (2021)