IL-12 (human) (rec.) (His)

CHF 270.00
In stock
CHI-HF-20112-C01010 µgCHF 270.00
CHI-HF-20112-C05050 µgCHF 810.00
 
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Product Details
Synonyms Interleukin-12; IL-12p70; IL-12p75; Cytotoxic Lymphocyte Maturation Factor; CLMF; NKSF; Natural Killer Cell Stimulatory Factor; TSF; T-cell Stimulatory Factor
Product Type Protein
Properties
Source/Host HEK 293 cells
Sequence The human IL-12 complex composed of the p40 subunit (aa 23-328) and the p35 subunit (aa 57-253) is fused at the C-terminus through a (G3S)4 linker to a His-tag.
Crossreactivity Human
Biological Activity The ED50 was determined by the induction of IFN-γ from NK cells co-stimulated with IL-18 is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥1 x 106units/mg.
MW ~85kDa (SDS-PAGE)
Purity ≥95% (SDS-PAGE)
Endotoxin Content <0.01EU/μg protein (LAL test; Lonza).
Reconstitution Reconstitute 10µg vial with 100 µl sterile water to a concentration of 0.1mg/ml.
Reconstitute 50µg vial with 100 µl sterile water to a concentration of 0.5mg/ml.
Add 1X PBS to the desired protein concentration.
Formulation Lyophilized from 0.2μm-filtered solution in PBS.
Other Product Data NCBI reference NP_002178.2: IL-12 p40 subunit (human) NCBI reference NP_000873.2: IL-12 p35 subunit (human)
Declaration Manufactured by Chimerigen.
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice Avoid freeze/thaw cycles.
PBS containing at least 0.1% BSA should be used for further dilutions.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
MSDS Inquire
Product Specification Sheet
Datasheet Download PDF
Interleukin-12 (IL-12), also known as Natural Killer Cell Stimulatory Factor (NKSF) or Cytotoxic Lymphocyte Maturation Factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. IL-12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and Natural Killer (NK) cells. Some of these IL-12 activities include the induction of IFN-γ and TNF in resting and activated T and NK cells; the enhancement of cytotoxic activity of resting NK and T cells, the stimulation of resting T cell proliferation in the presence of a comitogen; and the enhancement of NK cell proliferation. Current evidence indicates that IL-12 is a key mediator of cellular immunity and induces the differentiation of Th1 cells from precursor T helper cells. Based on its activities, it has been suggested that IL-12 may have therapeutic potential as a vaccine adjuvant that promotes cellular immunity and as an antitumor and anti viral agent.
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