Cookie Policy: This site uses cookies to improve your experience. You can find out more about our use of cookies in our Privacy Policy. By continuing to browse this site you agree to our use of cookies.
Chimerigen
IL-12 (mouse):Fc (human) (rec.)
470
CHF
CHF 470.00
In stock
CHI-MF-11112-C02525 µgCHF 470.00
| Product Details | |
|---|---|
| Synonyms | IL12-p70; CLMF; Cytotoxic Lymphocyte Maturation Factor; NKSF; Natural Killer Cell Stimulatory Factor; TSF; T cell Stimulating Factor |
| Product Type | Protein |
| Properties | |
| Source/Host | CHO cells |
| Sequence |
The mouse IL-12 complex composed of the p40 subunit (aa 1-335) and the p35 subunit (aa 23-215) is fused through a polypeptide linker to the Fc region of human IgG1. |
| Crossreactivity | Mouse |
| MW | ~100-110kDa (SDS-PAGE, reducing conditions) |
| Purity | ≥98% (SDS-PAGE) |
| Endotoxin Content | <0.06EU/μg protein (LAL test; Lonza). |
| Reconstitution | Reconstitute at 100μg/ml in sterile PBS. |
| Formulation | Lyophilized from 0.2μm-filtered solution in PBS. |
| Protein Negative Control | |
| Other Product Data |
NCBI reference NP_032377.1: IL-12 (mouse) |
| Declaration | Manufactured by Chimerigen. |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. |
| Use/Stability |
Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
| Documents | |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
Interleukin-12 (IL-12), also known as Natural Killer Cell Stimulatory Factor (NKSF) or Cytotoxic Lymphocyte Maturation Factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. IL-12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and Natural Killer (NK) cells. Some of these IL-12 activities include the induction of IFN-γ and TNF in resting and activated T and NK cells; the enhancement of cytotoxic activity of resting NK and T cells, the stimulation of resting T cell proliferation in the presence of a comitogen; and the enhancement of NK cell proliferation. Current evidence indicates that IL-12 is a key mediator of cellular immunity and induces the differentiation of Th1 cells from precursor T helper cells. Based on its activities, it has been suggested that IL-12 may have therapeutic potential as a vaccine adjuvant that promotes cellular immunity and as an antitumor and anti viral agent.
Product References
- Interleukin-12p35 knockout promotes macrophage differentiation, aggravates vascular dysfunction, and elevates blood pressure in angiotensin II-infused mice: J. Ye, et al.; Cardiovasc. Res. 115, 1102 (2019)
Related Products
