Cookie Policy: This site uses cookies to improve your experience. You can find out more about our use of cookies in our Privacy Policy. By continuing to browse this site you agree to our use of cookies.
Chimerigen
IL-2 (human):Fc (human) (rec.) (non-lytic)
300
CHF
CHF 300.00
In stock
CHI-HF-22002-C05050 µgCHF 300.00
| Product Details | |
|---|---|
| Synonyms | Interleukin-2; Aldesleukin |
| Product Type | Protein |
| Properties | |
| Source/Host | CHO cells |
| Sequence |
The extracellular domain of human IL-2 (aa 21-153) is fused to the N-terminus of the Fc region of a mutant human IgG1. |
| Crossreactivity | Human |
| Biological Activity |
Shows the biological function of the IL-2 moiety and exerts a prolonged circulating half-life caused by the modified Fc domain. |
| Purity | ≥98% (SDS-PAGE) |
| Endotoxin Content | <1EU/mg protein (LAL test; Lonza). |
| Reconstitution |
Reconstitute in 50µl sterile water. Add 1X PBS to the desired protein concentration. |
| Formulation | Lyophilized from 0.2μm-filtered solution in PBS. |
| Protein Negative Control | |
| Other Product Data |
Non-lytic: Acts as a long lasting fusion protein which only binds to the receptor. Mutations to the complement (C1q) and FcgR I binding sites of the IgGs Fc fragment render the fusion proteins incapable of antibody directed cytotoxicity (ADCC) and complement directed cytotoxicity (CDC). |
| Declaration | Manufactured by Chimerigen. |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. |
| Use/Stability |
Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
Interleukin-2 (IL-2) is a 133 amino acid glycoprotein with one intramolecular disulfide bond and variable glycosylation. It is secreted by activated T cells and induces proliferation and maturation of activated T cells, natural killer cells, and lymphokine activated killer cells. IL-2 also stimulates proliferation of antibody-producing B cells, activates neutrophils, and induces mononuclear cells to secrete IFN-γ and TNF-α and -β. Moreover, studies have shown that IL-2 is required for activation-induced apoptosis, an important hemeostatic mechanism in the immune system, which is involved in the maintenance of peripheral tolerance to self-antigens.
Product References
- Augmentation of immune responses to HIV-1 and simian immunodeficiency virus DNA vaccines by IL-2/Ig plasmid administration in rhesus monkeys: D.H. Barouch, et al.; PNAS 97, 4192 (2000)
- Control of viremia and prevention of clinical AIDS in Rhesus monkeys by cytokine-augmented DNA vaccination: D.H. Barouch, et al.; Science 290, 486 (2000)
- Vaccine-elicited immune responses prevent clinical AIDS in SHIV(89.6P)-infected rhesus monkeys: D.H. Barouch, et al.; Immunol. Lett. 79, 57 (2001)
- Spontaneous Resolution of Acute Rejection and Tolerance Induction With IL-2 Fusion Protein in Vascularized Composite Allotransplantation: R. Jindal, et al.; Am. J. Transplant. 15, 1231 (2015)
Related Products
