IL-10 (mouse):Fc (mouse) (rec.) (non-lytic)
|Synonyms||Interleukin-10; Cytokine Sythesis Inhibitory Factor; CSIF|
The extracellular domain of mouse IL-10 (aa 1-178) is fused to the N-terminus of the Fc region of a mutant mouse IgG2a.
Shows the biological function of the IL-10 moiety and exerts a prolonged circulating half-life caused by the modified Fc domain.
|Endotoxin Content||<0.06EU/μg protein (LAL test; Lonza).|
|Reconstitution||Reconstitute at 100μg/ml in sterile PBS.|
|Formulation||Lyophilized from 0.2μm-filtered solution in PBS.|
|Protein Negative Control|
|Other Product Data||
Non-lytic: Acts as a long lasting fusion protein which only binds to the receptor. Mutations to the complement (C1q) and FcgR I binding sites of the IgGs Fc fragment render the fusion proteins incapable of antibody directed cytotoxicity (ADCC) and complement directed cytotoxicity (CDC).
|Declaration||Manufactured by Chimerigen.|
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||-20°C|
|Handling Advice||Avoid freeze/thaw cycles.|
Stable for at least 1 year after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
|Product Specification Sheet|
Interleukin-10 (IL-10) is a cytokine produced by activated Th2 cells, B cells, keratinocytes and monocytes/macrophages. In vitro murine and human IL-10 inhibits cytokine synthesis by Th1 cells, natural killer cells and monocytes/macrophages. Several studies have suggested the potential application of IL-10 as an anti-inflammatory agent in the treatment of septic shock and as an immunosuppressive agent in certain T cell mediated autoimmune diseases.
- Administration of noncytolytic IL-10/Fc in murine models of lipopolysaccharide-induced septic shock and allogeneic islet transplantation: X.X. Zheng, et al.; J. Immunol. 154, 5590 (1995)
- Recombinant IL-10 and IL-10/Fc treatment down-regulate egg antigen- specific delayed hypersensitivity reactions and egg granuloma formation in schistosomiasis: P.O. Flores-Villanueva, et al.; J. Immunol. 156, 3315 (1996)
- A noncytolytic IL-10/Fc fusion protein prevents diabetes, blocks autoimmunity, and promotes suppressor phenomena in NOD mice: X.X. Zheng, et al.; J. Immunol. 158, 4507 (1997)
- IL-10/Fc inhibits macrophage function and prolongs pancreatic islet xenograft survival: X. Feng, et al.; Transplantation 68, 1775 (1999)
- Critical role of Kupffer cell-derived IL-10 for host defense in septic peritonitis: K. Emmanuilidis, et al.; J. Immunol. 167, 3919 (2001)