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Chimerigen
IL-10 (mouse):Fc (mouse) (rec.) (non-lytic)
300
CHF
CHF 300.00
In stock
CHI-MF-12010-C01010 µgCHF 300.00
CHI-MF-12010-C05050 µgCHF 510.00
| Product Details | |
|---|---|
| Synonyms | Interleukin-10; Cytokine Synthesis Inhibitory Factor; CSIF |
| Product Type | Protein |
| Properties | |
| Source/Host | CHO cells |
| Sequence |
The extracellular domain of mouse IL-10 (aa 1-178) is fused to the N-terminus of the Fc region of a mutant mouse IgG2a. |
| Crossreactivity | Mouse |
| Biological Activity |
Shows the biological function of the IL-10 moiety and exerts a prolonged circulating half-life caused by the modified Fc domain. |
| Purity | ≥98% (SDS-PAGE) |
| Endotoxin Content | <0.06EU/μg protein (LAL test; Lonza). |
| Reconstitution | Reconstitute at 100μg/ml in sterile PBS. |
| Formulation | Lyophilized from 0.2μm-filtered solution in PBS. |
| Protein Negative Control | |
| Other Product Data |
Non-lytic: Acts as a long lasting fusion protein which only binds to the receptor. Mutations to the complement (C1q) and FcgR I binding sites of the IgGs Fc fragment render the fusion proteins incapable of antibody directed cytotoxicity (ADCC) and complement directed cytotoxicity (CDC). |
| Declaration | Manufactured by Chimerigen. |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. |
| Use/Stability |
Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
| Documents | |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
Interleukin-10 (IL-10) is a cytokine produced by activated Th2 cells, B cells, keratinocytes and monocytes/macrophages. In vitro murine and human IL-10 inhibits cytokine synthesis by Th1 cells, natural killer cells and monocytes/macrophages. Several studies have suggested the potential application of IL-10 as an anti-inflammatory agent in the treatment of septic shock and as an immunosuppressive agent in certain T cell mediated autoimmune diseases.
Product References
- Administration of noncytolytic IL-10/Fc in murine models of lipopolysaccharide-induced septic shock and allogeneic islet transplantation: X.X. Zheng, et al.; J. Immunol. 154, 5590 (1995)
- Recombinant IL-10 and IL-10/Fc treatment down-regulate egg antigen- specific delayed hypersensitivity reactions and egg granuloma formation in schistosomiasis: P.O. Flores-Villanueva, et al.; J. Immunol. 156, 3315 (1996)
- A noncytolytic IL-10/Fc fusion protein prevents diabetes, blocks autoimmunity, and promotes suppressor phenomena in NOD mice: X.X. Zheng, et al.; J. Immunol. 158, 4507 (1997)
- IL-10/Fc inhibits macrophage function and prolongs pancreatic islet xenograft survival: X. Feng, et al.; Transplantation 68, 1775 (1999)
- Critical role of Kupffer cell-derived IL-10 for host defense in septic peritonitis: K. Emmanuilidis, et al.; J. Immunol. 167, 3919 (2001)
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