IL-10 (mouse):Fc (mouse) (rec.) (non-lytic)

CHF 330.00
In stock
CHI-MF-12010-C01010 µgCHF 330.00
CHI-MF-12010-C05050 µgCHF 570.00
CHI-MF-12010-50105 x 10 µgCHF 1'155.00
More Information
Product Details
Synonyms Interleukin-10; Cytokine Sythesis Inhibitory Factor; CSIF
Product Type Protein
Properties
Source/Host CHO cells
Sequence

The extracellular domain of mouse IL-10 (aa 1-178) is fused to the N-terminus of the Fc region of a mutant mouse IgG2a.

Crossreactivity Mouse
Biological Activity

Shows the biological function of the IL-10 moiety and exerts a prolonged circulating half-life caused by the modified Fc domain.

Purity ≥98% (SDS-PAGE)
Endotoxin Content <0.06EU/μg protein (LAL test; Lonza).
Reconstitution Reconstitute at 100μg/ml in sterile PBS.
Formulation Lyophilized from 0.2μm-filtered solution in PBS.
Protein Negative Control

Fc (mouse) IgG2a Control (rec.) (non-lytic)

Other Product Data

Non-lytic: Acts as a long lasting fusion protein which only binds to the receptor. Mutations to the complement (C1q) and FcgR I binding sites of the IgGs Fc fragment render the fusion proteins incapable of antibody directed cytotoxicity (ADCC) and complement directed cytotoxicity (CDC).

NCBI reference AAI37845.1: IL-10 (mouse)

Declaration Manufactured by Chimerigen.
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
MSDS Inquire
Product Specification Sheet
Datasheet Download PDF

Interleukin-10 (IL-10) is a cytokine produced by activated Th2 cells, B cells, keratinocytes and monocytes/macrophages. In vitro murine and human IL-10 inhibits cytokine synthesis by Th1 cells, natural killer cells and monocytes/macrophages. Several studies have suggested the potential application of IL-10 as an anti-inflammatory agent in the treatment of septic shock and as an immunosuppressive agent in certain T cell mediated autoimmune diseases.

Product References
  1. Administration of noncytolytic IL-10/Fc in murine models of lipopolysaccharide-induced septic shock and allogeneic islet transplantation: X.X. Zheng, et al.; J. Immunol. 154, 5590 (1995)
  2. Recombinant IL-10 and IL-10/Fc treatment down-regulate egg antigen- specific delayed hypersensitivity reactions and egg granuloma formation in schistosomiasis: P.O. Flores-Villanueva, et al.; J. Immunol. 156, 3315 (1996)
  3. A noncytolytic IL-10/Fc fusion protein prevents diabetes, blocks autoimmunity, and promotes suppressor phenomena in NOD mice: X.X. Zheng, et al.; J. Immunol. 158, 4507 (1997)
  4. IL-10/Fc inhibits macrophage function and prolongs pancreatic islet xenograft survival: X. Feng, et al.; Transplantation 68, 1775 (1999)
  5. Critical role of Kupffer cell-derived IL-10 for host defense in septic peritonitis: K. Emmanuilidis, et al.; J. Immunol. 167, 3919 (2001)
© 2017 Adipogen Life Sciences. Pictures: © 2012 Martin Oeggerli. All Rights Reserved.