anti-Dibromo-tyrosine [DiBrY], mAb (3A5)

CHF 240.00
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JAI-MBY-020P20 µgCHF 240.00
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Product Details
Product Type Monoclonal Antibody
Clone 3A5
Isotype Mouse IgG1κ
Immunogen/Antigen Brominated keyhole limpet hemocyanin.

Immunohistochemistry: Recommended concentration is 10μg/mL
Western Blot

Crossreactivity All

Recognizes dibromotyrosine (DiBrY), N-acetylated dichloro tyrosine (NAcDiClY), diiodo tyrosine (DiIY), N-bromosuccinimide-treated BSA, HOCl-treated BSA and dibromo-tyrosone conjugated BSA (halogene-modified tyrosine at both 3- and 5-position). Does not cross-react with dityrosine-conjugated BSA, peroxynitrite-treated BSA, tyrosine (Y), chrolotyrosine(ClY), 3-3’-dityrosine (DiY), 3-aminotyrosine (NH2Y), 3-nitrotyrosine (NO2Y), phenylalanine (Phe), o-phosphotyrosine, 3,4-dihydroxyphenylacetic acid (DOPA) and 2-hydroxy-4-phenylacetic acid. Weakly cross-reacts with Bromotyrosine (BrY).

Purity Detail Protein A purified.
Formulation Lyophilized. Contains 10mM Phosphate buffered saline (pH7.4).
Reconstitution Reconstitute with 200μL of distilled water.
Isotype Negative Control

Mouse IgG1 Isotype Control

Other Product Data

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Our product description may differ slightly from the original manufacturers product datasheet.

Declaration Manufactured by JaICA.
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice Avoid freeze/thaw cycles.
Use/Stability Stable for at least 3 years after receipt when stored at -20°C.
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Product Specification Sheet
Datasheet Download PDF

Neutrophils and eosinophils play an important role in the defence system against microbial infection. Myeloperoxidase (MPO) and eosinophil peroxidase (EPO) are known to catalyze formation of hypochlorous acid (HOCl) and hypobromous acid (HOBr). These reactive intermediates may react with proteins, lipids and nucleotides, and it has been reported to form tyrosine halogenation such as dibromotyrosine (DiBrY). DiBrY is a Br-modified tyrosine at 3- and 5- position, which is one of the major oxidative products derived from neutrophil myeloperoxidase. DiBrY may be a novel biomarker for tissue damage which is related to inflammatory and allergic disorders.

Product References
  1. Immunogenicity of a brominated protein and successive establishment of a monoclonal antibody to dihalogenated tyrosine: Y. Kato, et al.; Free Rad. Biol. Med. 38, 24 (2005)
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