anti-Dibromo-tyrosine [DiBrY], mAb (3A5)
JAI-MBY-020P20 µgCHF 239.00
Immunohistochemical staining using anti-Dibromo-tyrosine, mAb (3A5).
|Product Type||Monoclonal Antibody|
|Immunogen/Antigen||Brominated keyhole limpet hemocyanin.|
Immunohistochemistry: Recommended concentration is 10μg/mL
|Specificity||Recognizes dibromotyrosine (DiBrY), N-acetylated dichloro tyrosine (NAcDiClY), diiodo tyrosine (DiIY), N-bromosuccinimide-treated BSA, HOCl-treated BSA and dibromo-tyrosone conjugated BSA (halogene-modified tyrosine at both 3- and 5-position). Does not cross-react with dityrosine-conjugated BSA, peroxynitrite-treated BSA, tyrosine (Y), chrolotyrosine(ClY), 3-3’-dityrosine (DiY), 3-aminotyrosine (NH2Y), 3-nitrotyrosine (NO2Y), phenylalanine (Phe), o-phosphotyrosine, 3,4-dihydroxyphenylacetic acid (DOPA) and 2-hydroxy-4-phenylacetic acid. Weakly cross-reacts with Bromotyrosine (BrY).|
|Purity Detail||Protein A purified.|
|Formulation||Lyophilized. Contains 10mM Phosphate buffered saline (pH7.4).|
|Reconstitution||Reconstitute with 200μL of distilled water.|
|Other Product Data||
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Our product description may differ slightly from the original manufacturers product datasheet.
|Declaration||Manufactured by JaICA.|
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||-20°C|
|Handling Advice||Avoid freeze/thaw cycles.|
|Use/Stability||Stable for at least 3 years after receipt when stored at -20°C.|
|Product Specification Sheet|
Neutrophils and eosinophils play an important role in the defence system against microbial infection. Myeloperoxidase (MPO) and eosinophil peroxidase (EPO) are known to catalyze formation of hypochlorous acid (HOCl) and hypobromous acid (HOBr). These reactive intermediates may react with proteins, lipids and nucleotides, and it has been reported to form tyrosine halogenation such as dibromotyrosine (DiBrY). DiBrY is a Br-modified tyrosine at 3- and 5- position, which is one of the major oxidative products derived from neutrophil myeloperoxidase. DiBrY may be a novel biomarker for tissue damage which is related to inflammatory and allergic disorders.
- Immunogenicity of a brominated protein and successive establishment of a monoclonal antibody to dihalogenated tyrosine: Y. Kato, et al.; Free Rad. Biol. Med. 38, 24 (2005)