JaICA

anti-Dibromo-tyrosine [DiBrY], mAb (3A5)

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JAI-MBY-020P20 µgCHF 240.00

Figure 1: Immunohistochemical staining using anti-Dibromo-tyrosine, mAb (3A5).

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Product Details
Product Type	Monoclonal Antibody
Properties
Clone	3A5
Isotype	Mouse IgG1κ
Immunogen/Antigen	Brominated keyhole limpet hemocyanin.
Application	ELISA Immunohistochemistry: Recommended concentration is 10μg/mL Western Blot
Crossreactivity	All Human
Specificity	Recognizes dibromotyrosine (DiBrY), N-acetylated dichloro tyrosine (NAcDiClY), diiodo tyrosine (DiIY), N-bromosuccinimide-treated BSA, HOCl-treated BSA and dibromo-tyrosone conjugated BSA (halogene-modified tyrosine at both 3- and 5-position). Does not cross-react with dityrosine-conjugated BSA, peroxynitrite-treated BSA, tyrosine (Y), chrolotyrosine(ClY), 3-3’-dityrosine (DiY), 3-aminotyrosine (NH2Y), 3-nitrotyrosine (NO2Y), phenylalanine (Phe), o-phosphotyrosine, 3,4-dihydroxyphenylacetic acid (DOPA) and 2-hydroxy-4-phenylacetic acid. Weakly cross-reacts with Bromotyrosine (BrY).
Purity Detail	Protein A purified.
Formulation	Lyophilized. Contains 10mM Phosphate buffered saline (pH7.4).
Reconstitution	Reconstitute with 200μL of distilled water.
Isotype Negative Control	Mouse IgG1 Isotype Control
Other Product Data	Click here for Original Manufacturer Product Datasheet Our product description may differ slightly from the original manufacturers product datasheet.
Declaration	Manufactured by JaICA.
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	Avoid freeze/thaw cycles.
Use/Stability	Stable for at least 3 years after receipt when stored at -20°C.
Documents
Protocols	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

Neutrophils and eosinophils play an important role in the defence system against microbial infection. Myeloperoxidase (MPO) and eosinophil peroxidase (EPO) are known to catalyze formation of hypochlorous acid (HOCl) and hypobromous acid (HOBr). These reactive intermediates may react with proteins, lipids and nucleotides, and it has been reported to form tyrosine halogenation such as dibromotyrosine (DiBrY). DiBrY is a Br-modified tyrosine at 3- and 5- position, which is one of the major oxidative products derived from neutrophil myeloperoxidase. DiBrY may be a novel biomarker for tissue damage which is related to inflammatory and allergic disorders.

Product References

Immunogenicity of a brominated protein and successive establishment of a monoclonal antibody to dihalogenated tyrosine: Y. Kato, et al.; Free Rad. Biol. Med. 38, 24 (2005)

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