anti-4-Hydroxy-2-nonenal [4-HNE], mAb (HNEJ-2)

CHF 698.00
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JAI-MHN-100P100 µgCHF 698.00
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Product Details
Product Type Monoclonal Antibody
Clone HNEJ-2
Isotype Mouse IgG1κ
Source/Host Ascites
Immunogen/Antigen 4-Hydroxy-2-nonenal (4-HNE) modified keyhole limpet hemocyanin.

Immunohistochemistry: Recommended concentration is 25μg/mL
Western Blot: Recommended concentration is 15μg/mL

Crossreactivity All

Recognizes 4-HNE-lysine, 4-HNE-histidine and 4-HNE cysteine adduct. Has much higher affinity for the 4-HNE-histidine adduct than 4-HNE-lysine or 4-HNE cysteine adduct. Shows almost negligible reactivity with proteins that were treated with other aldehydes, such as 2-nonenal, 2-hexenal, 1-hexanal, 4-hydroxy-2-hexenal, formaldehyde, or glutaraldehyde.

Purity Detail Ammonium sulfate purified.
Formulation Lyophilized. Contains 50mM Tris-buffered saline (TBS).
Reconstitution Reconstitute with 1000μL of distilled water.
Isotype Negative Control

Mouse IgG1 Isotype Control

Other Product Data

Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.

Declaration Manufactured by JaICA.
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice Avoid freeze/thaw cycles.
Use/Stability Stable for at least 3 years after receipt when stored at -20°C.
After reconstitution, prepare aliquots and store at -20°C.
Protocols Download PDF
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Product Specification Sheet
Datasheet Download PDF

Reactive oxygen species (ROS) are involved in lipid peroxidation inside the living bodies, and membrane lipids are one of the major targets of ROS. During the peroxidation process, a variery of aldehydes are formed. 4-hydroxy-2-nonenal (4-HNE) is an alpha, beta unsaturated aldehyde that can be formed by peroxidation of omega-6 unsaturated fatty acids such as linoleic acid and arachidonic acid. Especially, 4-HNE formed inside living body is reported to originate from phospholipid-bound arachidonic acid. 4-HNE may be one of the most reliable biomarker of lipid peroxidation.

Product References
  1. The monclonal antibody specific for the 4-hydroxy-2-nonenal histidine adduct: S.Toyokuni, et al.; FEBS Lett. 359, 189 (1995) [Western blot] 
  2. Induction and nuclear translocation of thioredoxin by oxidative damage in the mouse kidney: independence of tubular necrosis and sulfhydryl depletion: T. Tanaka, et al.; Lab. Invest. 77, 145 (1997)
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