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JaICA
anti-4-Hydroxy-2-nonenal [4-HNE], mAb (HNEJ-2)
180
CHF
CHF 180.00
In stock
JAI-MHN-020P20 µgCHF 180.00
Figure 1: High-power view of colorectal carcinoma cells using anti-4-HNE, mAb (HNEJ-2). Adenocarcinoma (arrows) and nontumorous epithelial cells (star x240).
| Product Details | |
|---|---|
| Product Type | Monoclonal Antibody |
| Properties | |
| Clone | HNEJ-2 |
| Isotype | Mouse IgG1κ |
| Source/Host | Ascites |
| Immunogen/Antigen | 4-Hydroxy-2-nonenal (4-HNE) modified keyhole limpet hemocyanin. |
| Application |
Immunohistochemistry: Recommended concentration is 25μg/mL |
| Crossreactivity |
All Human |
| Specificity |
Recognizes 4-HNE-lysine, 4-HNE-histidine and 4-HNE cysteine adduct. Has much higher affinity for the 4-HNE-histidine adduct than 4-HNE-lysine or 4-HNE cysteine adduct. Shows almost negligible reactivity with proteins that were treated with other aldehydes, such as 2-nonenal, 2-hexenal, 1-hexanal, 4-hydroxy-2-hexenal, formaldehyde, or glutaraldehyde. |
| Purity Detail | Ammonium sulfate purified. |
| Formulation | Lyophilized. Contains 50mM Tris-buffered saline (TBS). |
| Reconstitution | Reconstitute with 200μL of distilled water. |
| Isotype Negative Control | |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| Declaration | Manufactured by JaICA. |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Avoid freeze/thaw cycles. |
| Use/Stability |
Stable for at least 2 years after receipt when stored at -20°C. After reconstitution, prepare aliquots and store at -20°C. |
| Documents | |
| Protocols |
 Download PDF |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
Reactive oxygen species (ROS) are involved in lipid peroxidation inside the living bodies, and membrane lipids are one of the major targets of ROS. During the peroxidation process, a variery of aldehydes are formed. 4-hydroxy-2-nonenal (4-HNE) is an alpha, beta unsaturated aldehyde that can be formed by peroxidation of omega-6 unsaturated fatty acids such as linoleic acid and arachidonic acid. Especially, 4-HNE formed inside living body is reported to originate from phospholipid-bound arachidonic acid. 4-HNE may be one of the most reliable biomarker of lipid peroxidation.
Product References
- The monclonal antibody specific for the 4-hydroxy-2-nonenal histidine adduct: S.Toyokuni, et al.; FEBS Lett. 359, 189 (1995) [Western blot]
- Induction and nuclear translocation of thioredoxin by oxidative damage in the mouse kidney: independence of tubular necrosis and sulfhydryl depletion: T. Tanaka, et al.; Lab. Invest. 77, 145 (1997)
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